Danger J M, Benyamina M, Guy J, Leboulenger F, Tonon M C, Pelletier G, Vaudry H
Ann Endocrinol (Paris). 1986;47(1):59-61.
A neuropeptide Y (NPY)-like peptide has been localized in the frog brain by the direct immunofluorescence method. Subcellular localization of NPY has been studied by PAP immunocytochemistry and the immunogold technique. Using a specific radioimmunoassay technique, NPY-like peptide has been quantified in various brain areas and the immunoreactive material was characterized by Sephadex G-50 gel filtration and high performance liquid chromatography. The results indicate that frog NPY is closely related to the porcine molecule and largely distributed in the frog brain. This peptide may play both neurotransmitter and neuroendocrine functions in amphibia.
一种神经肽Y（NPY）样肽已通过直接免疫荧光法在蛙脑中定位。通过PAP免疫细胞化学和免疫金技术研究了NPY的亚细胞定位。使用特定的放射免疫测定技术，已对蛙脑各个区域的NPY样肽进行了定量，并通过Sephadex G-50凝胶过滤和高效液相色谱对免疫反应性物质进行了表征。结果表明，蛙NPY与猪分子密切相关，且在蛙脑中广泛分布。这种肽可能在两栖动物中发挥神经递质和神经内分泌功能。
