Pilar-Orive Francisco J, Astigarraga Itziar, Azkargorta Mikel, Elortza Felix, Garcia-Obregon Susana
Pediatric Critical Care Group, Biocruces Bizkaia Health Research Institute, 48903 Barakaldo, Spain.
Pediatric Critical Care Service, Hospital Universitario Cruces, 48903 Barakaldo, Spain.
J Clin Med. 2022 Mar 12;11(6):1563. doi: 10.3390/jcm11061563.
Sepsis is a syndrome without a standard validated diagnostic test. Early recognition is crucial. Serum proteome analysis in children with sepsis may identify new biomarkers. This study aimed to find suitable blood biomarkers for an early diagnosis of sepsis. An analytical observational case-control study was carried out in a single center. Children admitted to a Pediatric Intensive Care Unit with clinical diagnosed sepsis were eligible for study. A proteomic analysis conducted by mass spectrometry was performed. Forty patients with sepsis and 24 healthy donors were recruited. Proteomics results revealed 44 proteins differentially expressed between patients and healthy controls. Six proteins were selected to be validated: lactoferrin, serum amyloid-A1 (SAA-1), complement factor B, leucine-rich alpha-2 glycoprotein (LRG1), soluble interleukin-2 alpha chain receptor (sCD25) and soluble haptoglobin−hemoglobin receptor. Our results showed that sCD25, SAA-1, and LRG1 had high levels of specificity and sensitivity, as well as an excellent area under the ROC curve (>0.9). Our study provides a serum proteomic analysis that identifies new diagnostic biomarkers in sepsis. SAA-1, sCD25 and LRG1 were able to separate septic from healthy donor, so they could be used together with other clinical and analytical features to improve sepsis diagnosis in children.
脓毒症是一种没有经过标准验证诊断测试的综合征。早期识别至关重要。脓毒症患儿的血清蛋白质组分析可能会识别出新的生物标志物。本研究旨在寻找适合早期诊断脓毒症的血液生物标志物。在一个单一中心开展了一项分析性观察性病例对照研究。入住儿科重症监护病房且临床诊断为脓毒症的儿童符合研究条件。进行了质谱蛋白质组分析。招募了40例脓毒症患者和24名健康供者。蛋白质组学结果显示患者与健康对照之间有44种蛋白质表达存在差异。选择了6种蛋白质进行验证：乳铁蛋白、血清淀粉样蛋白A1（SAA-1）、补体因子B、富含亮氨酸的α-2糖蛋白（LRG1）、可溶性白细胞介素-2α链受体（sCD25）和可溶性触珠蛋白-血红蛋白受体。我们的结果表明，sCD25、SAA-1和LRG1具有高特异性和敏感性，以及优异的ROC曲线下面积（>0.9）。我们的研究提供了一种血清蛋白质组分析，可识别脓毒症中新的诊断生物标志物。SAA-1、sCD25和LRG1能够区分脓毒症患者与健康供者，因此它们可与其他临床和分析特征一起用于改善儿童脓毒症的诊断。
