Li Yaxing, Tang Haohao, Zhao Weichao, Yang Yang, Fan Xiaolu, Zhan Guanping, Li Jiahuan, Sun Shujing
College of Life Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Basic Forestry and Proteomics Research Center, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
J Fungi (Basel). 2022 Feb 28;8(3):242. doi: 10.3390/jof8030242.
is a dimorphic fungus that can undertake a reversible transition between yeast-like conidia and hyphal forms. The transformation mechanism and proteomic differences between these two forms have not been reported. Therefore, in this study, we attempted to explore the differential protein profiles of dikaryotic yeast-like conidia from fruiting bodies and mycelia (FBMds) and dikaryotic mycelia (DM) by synthetically applying high-resolution MS1-based quantitative data-independent acquisition (HRMS1-DIA) full proteomics and parallel reaction monitoring (PRM) targeted proteomics. The results showed that a total of 5687 proteins were quantified, and 2220 of them (39.01%) showed more than a two-fold change in expression. The functional analysis of the differentially expressed proteins (DEPs) confirmed that the DEPs were mainly located in the membrane and nucleus. The FBMds tended to express proteins involved in biosynthesis, metabolism, DNA replication and transcription, and DNA damage repair. At the same time, DM exhibited an increased expression of proteins involved in signal transduction mechanisms such as the mitogen-activated protein kinase (MAPK) signaling pathway and the Ras signaling pathway. Further, phosphorylation analysis confirmed the importance of the MAPK signaling pathway in dimorphism, and comparative metabolism analysis demonstrated the metabolic difference between FBMds and DM. The information obtained in the present study will provide new insights into the difference between FBMds and DM and lay a foundation for further research on the dimorphism formation mechanism of .
是一种双态真菌，可在酵母样分生孢子和菌丝形态之间进行可逆转变。这两种形态之间的转化机制和蛋白质组学差异尚未见报道。因此，在本研究中，我们综合应用基于高分辨率MS1的定量数据非依赖采集（HRMS1-DIA）全蛋白质组学和平行反应监测（PRM）靶向蛋白质组学，试图探索来自子实体和菌丝体的双核酵母样分生孢子（FBMds）和双核菌丝体（DM）的差异蛋白质谱。结果表明，共鉴定出5687种蛋白质，其中2220种（39.01%）表达变化超过两倍。对差异表达蛋白（DEPs）的功能分析证实，这些蛋白主要位于细胞膜和细胞核。FBMds倾向于表达参与生物合成、代谢、DNA复制和转录以及DNA损伤修复的蛋白质。同时，DM中参与丝裂原活化蛋白激酶（MAPK）信号通路和Ras信号通路等信号转导机制的蛋白质表达增加。此外，磷酸化分析证实了MAPK信号通路在双态性中的重要性，比较代谢分析揭示了FBMds和DM之间的代谢差异。本研究获得的信息将为深入了解FBMds和DM之间的差异提供新的见解，并为进一步研究其双态性形成机制奠定基础。
