Van Hooff C O, De Graan P N, Boonstra J, Oestreicher A B, Schmidt-Michels M H, Gispen W H
Biochem Biophys Res Commun. 1986 Sep 14;139(2):644-51. doi: 10.1016/s0006-291x(86)80039-0.
Exposure of PC12 cells to nerve growth factor results in arrest of cell growth and induction of differentiation to sympathetic neuron-like cells, bearing neurites. In this study we identify a 48 kDa PC12 phosphoprotein as the neuron-specific protein kinase C substrate B-50 (Mr 48 kDa; IEP 4.5) on basis of comigration with purified B-50, immunoreactivity and phosphopeptide mapping. B-50 is present in both undifferentiated and differentiated PC12 cells. Exposure of PC12 cells to nerve growth factor for two days results in a 2.5-fold increase in the amount of B-50 as measured by RIA. Indirect immunofluorescence microscopy reveals that B-50 is mainly localized at the cell membrane and in growth cones. Our data are in line with the hypothesis that B-50 plays a role in neurite outgrowth and indicate that PC12 cells provide a suitable model to study this hypothesis.
将PC12细胞暴露于神经生长因子会导致细胞生长停滞，并诱导其分化为带有神经突的交感神经元样细胞。在本研究中，我们基于与纯化的B-50共迁移、免疫反应性和磷酸肽图谱，鉴定出一种48 kDa的PC12磷蛋白为神经元特异性蛋白激酶C底物B-50（分子量48 kDa；等电点4.5）。B-50存在于未分化和分化的PC12细胞中。通过放射免疫分析测定，将PC12细胞暴露于神经生长因子两天后，B-50的量增加了2.5倍。间接免疫荧光显微镜检查显示，B-50主要定位于细胞膜和生长锥。我们的数据与B-50在神经突生长中起作用的假设一致，并表明PC12细胞为研究该假设提供了一个合适的模型。
