5,6-环氧二十碳三烯酸可动员垂体前叶细胞中的钙离子。

5,6-Epoxyeicosatrienoic acid mobilizes Ca2+ in anterior pituitary cells.

作者信息

Snyder G, Lattanzio F, Yadagiri P, Falck J R, Capdevila J

出版信息

Biochem Biophys Res Commun. 1986 Sep 30;139(3):1188-94. doi: 10.1016/s0006-291x(86)80303-5.

Abstract

Luteinizing hormone releasing hormone stimulates the concomitant release of luteinizing hormone and 45Ca2+ from prelabeled anterior pituitary cells. Indomethacin (10 microM) and nordihydroguaiaretic acid (10 microM) had no effect on the luteinizing hormone releasing hormone-stimulated release of either luteinizing hormone or 45Ca2+. Eicosatetraynoic acid (10 microM) blocked both luteinizing hormone releasing hormone-stimulated luteinizing hormone secretion and luteinizing hormone releasing hormone-stimulated 45Ca2+ efflux. 5,6-Epoxyeicosatrienoic acid stimulated both luteinizing hormone secretion and 45Ca2+ efflux from anterior pituitary cells. Additionally, 5,6-epoxyeicosatrienoic acid closely mimics the ability of luteinizing hormone releasing hormone to increase intracellular free calcium. These results are consistent with the hypothesis that 5,6-EET alters calcium homeostasis in a manner similar to that observed during luteinizing hormone releasing hormone stimulation of luteinizing hormone release.

摘要

促黄体生成素释放激素刺激预先标记的垂体前叶细胞同时释放促黄体生成素和45Ca2+。吲哚美辛（10微摩尔）和去甲二氢愈创木酸（10微摩尔）对促黄体生成素释放激素刺激的促黄体生成素或45Ca2+释放均无影响。二十碳四炔酸（10微摩尔）阻断了促黄体生成素释放激素刺激的促黄体生成素分泌以及促黄体生成素释放激素刺激的45Ca2+外流。5,6-环氧二十碳三烯酸刺激垂体前叶细胞的促黄体生成素分泌和45Ca2+外流。此外，5,6-环氧二十碳三烯酸紧密模拟促黄体生成素释放激素增加细胞内游离钙的能力。这些结果与以下假设一致，即5,6-环氧二十碳三烯酸以类似于促黄体生成素释放激素刺激促黄体生成素释放时观察到的方式改变钙稳态。