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蓝莓(越桔属)果实内生菌:通过无标记表面增强拉曼光谱(SERS)对酵母和细菌进行表征。

Endophytes from blueberry (Vaccinium sp.) fruit: Characterization of yeast and bacteria via label-free surface-enhanced Raman spectroscopy (SERS).

作者信息

Vaitiekūnaitė Dorotėja, Bružaitė Ingrida, Snitka Valentinas

机构信息

Lithuanian Research Centre for Agriculture and Forestry, Laboratory of Forest Plant Biotechnology, Institute of Forestry, Liepu st. 1, LT-53101 Girionys, Lithuania.

Department of Chemistry and Bioengineering, Faculty of Fundamental Sciences, Vilnius Gediminas Technical University, Sauletekio av. 11, LT-10223 Vilnius, Lithuania.

出版信息

Spectrochim Acta A Mol Biomol Spectrosc. 2022 Jul 5;275:121158. doi: 10.1016/j.saa.2022.121158. Epub 2022 Mar 15.

Abstract

Blueberries (Vaccinium sp.) are consumed all around the globe, however, their endophytic community has not been thoroughly researched, specifically their fruit endophytes. We aimed to isolate and analyze easily cultivable blueberry fruit endophytes to help in future research, concerning probiotic microorganisms. Twelve strains were isolated in this pilot study, genetically homologous with Staphylococcus hominis, Staphylococcus cohnii, Salmonella enterica, Leuconostoc mesenteroides, and [Candida] santamariae. To determine the molecular composition of these isolates we used label-free surface-enhanced Raman spectroscopy (SERS). To our knowledge, this is the first time that SERS spectra for L. mesenteroides and C. santamariae are presented, as well as the first report of Candida yeast, isolated specifically from blueberry fruits. Our findings suggest that the differences in tested yeast and bacteria SERS spectra and subsequent differentiation are facilitated by minor shifts in spectral peak positions as well as their intensities. Moreover, we used principal component and discriminant function analyses to differentiate chemotypes within our isolate group, proving the sensitivity of the technique and its usefulness to recognize different strains in plant-associated microbe samples, which will aid to streamline future studies in biofertilizers and biocontrol agents.

摘要

蓝莓(越橘属)在全球范围内都有食用,然而,其内生菌群落尚未得到充分研究,特别是其果实内生菌。我们旨在分离和分析易于培养的蓝莓果实内生菌,以助力未来有关益生菌微生物的研究。在这项初步研究中分离出了12株菌株,它们在基因上与霍氏葡萄球菌、科氏葡萄球菌、肠炎沙门氏菌、肠系膜明串珠菌和圣玛丽亚假丝酵母同源。为了确定这些分离株的分子组成,我们使用了无标记表面增强拉曼光谱(SERS)。据我们所知,这是首次展示肠系膜明串珠菌和圣玛丽亚假丝酵母的SERS光谱,也是首次报道从蓝莓果实中特异性分离出的假丝酵母。我们的研究结果表明,测试的酵母和细菌SERS光谱的差异以及随后的区分是由光谱峰位置及其强度的微小变化促成的。此外,我们使用主成分分析和判别函数分析来区分分离株组内的化学型,证明了该技术的敏感性及其在识别植物相关微生物样品中不同菌株方面的有用性,这将有助于简化未来在生物肥料和生物防治剂方面的研究。

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