Mažeikienė Ingrida, Frercks Birutė, Kurgonaitė Monika, Rasiukevičiūtė Neringa, Mačionienė Irena
Department of Orchard Plant Genetics and Biotechnology, Institute of Horticulture, Lithuanian Research Centre for Agriculture and Forestry, Kaunas District, LT-54333 Babtai, Lithuania.
Research Laboratory of Microbiology, Food Institute, Kaunas University of Technology, Radvilėnų Avenue 19, LT-50254 Kaunas, Lithuania.
Int J Mol Sci. 2025 Jul 11;26(14):6677. doi: 10.3390/ijms26146677.
Plant microbiota contributes to nutrient absorption, and the production of hormones and vitamins, and plays a crucial role in responding to environmental stress. We hypothesized that spp. harbour a unique microbiota that enables them to coexist in extreme environments such as saline, nutrient-poor, and waterlogged conditions. Upon examining spp. endophytes isolated from blueberries, cranberries and lingonberries in vitro, we identified (Bil-LT1_1, Bil-LT1_2) and (Cran-LT1_8, Ling-NOR4_15) strains that inhibit the growth of five pathogenic fungi and five foodborne bacteria. Whole-genome sequencing provided insights into genome organization and plasticity, helping identify mobile elements and genes potentially acquired through horizontal gene transfer. Functional annotation identified genes associated with plant colonization, stress tolerance, biocontrol activity, and plant growth promotion. Comparative genomic analyses revealed key biosynthetic gene clusters (BGCs) responsible for producing antifungal metabolites, including lipopeptides and polyketides. Genes supporting plant nutrition, growth, and environmental adaptation were present also in these strains. Notably, isolated endophytes exhibited particularly high levels of genomic plasticity, likely due to horizontal gene transfer involving gene ontology (GO) pathways related to survival in polymicrobial and foreign environments.
植物微生物群有助于养分吸收、激素和维生素的产生，并在应对环境胁迫中发挥关键作用。我们假设某物种拥有独特的微生物群，使其能够在极端环境（如盐碱地、营养贫瘠和涝渍条件）中共存。在体外检查从蓝莓、蔓越莓和越橘中分离出的某物种内生菌后，我们鉴定出了抑制五种致病真菌和五种食源细菌生长的菌株（Bil-LT1_1、Bil-LT1_2）和（Cran-LT1_8、Ling-NOR4_15）。全基因组测序提供了对基因组组织和可塑性的见解，有助于识别可能通过水平基因转移获得的移动元件和基因。功能注释确定了与植物定殖、胁迫耐受性、生物防治活性和植物生长促进相关的基因。比较基因组分析揭示了负责产生抗真菌代谢物（包括脂肽和聚酮化合物）的关键生物合成基因簇（BGC）。支持植物营养、生长和环境适应的基因也存在于这些菌株中。值得注意的是，分离出的内生菌表现出特别高的基因组可塑性，这可能是由于涉及与多微生物和外来环境中生存相关的基因本体（GO）途径的水平基因转移。
