3',4'-二羟基苯乙二醇对 1 型糖尿病样大鼠的神经保护作用——羟基酪醇/3',4'-二羟基苯乙二醇比值的影响。
Neuroprotective Effect of 3',4'-Dihydroxyphenylglycol in Type-1-like Diabetic Rats-Influence of the Hydroxytyrosol/3',4'-dihydroxyphenylglycol Ratio.
机构信息
Department of Pharmacology, Biomedical Research Institute (IBIMA), School of Medicine, University of Málaga, 29010 Málaga, Spain.
Clinical Laboratory Department, Public Hospital, Montilla, 14550 Córdoba, Spain.
出版信息
Nutrients. 2022 Mar 8;14(6):1146. doi: 10.3390/nu14061146.
The aim of this study was to assess the possible neuroprotective effect of 3',4'-dihydroxyphenylglycol (DHPG), a polyphenol from extra virgin olive oil (EVOO), in an experimental model of diabetes and whether this effect is modified by the presence of another EVOO polyphenol, hydroxytyrosol (HT). The neuroprotective effect was assessed in a hypoxia-reoxygenation model in brain slices and by quantifying retinal nerve cells. The animals were distributed as follows: (1) normoglycemic rats (NDR), (2) diabetic rats (DR), (3) DR treated with HT (5 mg/kg/day p.o.), (4) DR treated with DHPG (0.5 mg/kg/day), or (5) with 1 mg/kg/day, (6) DR treated with HT plus DHPG 0.5 mg/kg/day, or (7) HT plus 1 mg/kg/day p.o. DHPG. Diabetic animals presented higher levels of oxidative stress variables and lower numbers of neuronal cells in retinal tissue. The administration of DHPG or HT reduced most of the oxidative stress variables and brain lactate dehydrogenase efflux (LDH) as an indirect index of cellular death and also reduced the loss of retinal cells. The association of DHPG+HT in the same proportions, as found in EVOO, improved the neuroprotective and antioxidant effects of both polyphenols.
本研究旨在评估 3'，4'-二羟基苯乙二醇（DHPG）——一种特级初榨橄榄油（EVOO）中的多酚——在糖尿病实验模型中的可能神经保护作用，以及这种作用是否会受到另一种 EVOO 多酚——羟基酪醇（HT）的影响。在脑切片的缺氧再复氧模型中以及通过定量视网膜神经细胞来评估神经保护作用。将动物分为以下几类：（1）正常血糖大鼠（NDR），（2）糖尿病大鼠（DR），（3）用 HT（5mg/kg/天，口服）治疗的 DR，（4）用 DHPG（0.5mg/kg/天）治疗的 DR，（5）用 1mg/kg/天，（6）用 HT 加 DHPG 0.5mg/kg/天，或（7）HT 加 1mg/kg/天，口服。糖尿病动物的氧化应激变量水平更高，视网膜组织中的神经元细胞数量更少。DHPG 或 HT 的给药降低了大多数氧化应激变量和脑乳酸脱氢酶（LDH）的流出（作为细胞死亡的间接指标），并减少了视网膜细胞的损失。以与 EVOO 中相同的比例联合使用 DHPG+HT 可改善这两种多酚的神经保护和抗氧化作用。
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