González-Correa José Antonio, Navas María Dolores, Lopez-Villodres Juan Antonio, Trujillo Mariana, Espartero José Luis, De La Cruz José Pedro
Laboratorio de Investigaciones Antitrombóticas e Isquemia Tisular (LIAIT), Department of Pharmacology, School of Medicine, University of Malaga, Campus de Teatinos s/n, 29071 Malaga, Spain.
Neurosci Lett. 2008 Dec 3;446(2-3):143-6. doi: 10.1016/j.neulet.2008.09.022.
Hydroxytyrosol (HT) and hydroxytyrosol acetate (HT-AC) are two well-known phenolic compounds with antioxidant properties that are present in virgin olive oil (VOO). Because VOO has shown neuroprotective effects in rats, the purpose of the present study was to investigate the possible neuroprotective effect of HT and HT-AC in a model of hypoxia-reoxygenation in rat brain slices after in vitro incubation of these compounds or after 7 days of oral treatment with 5 or 10 mg/kg per day. Lactate dehydrogenase (LDH) efflux to the incubation medium was measured as a marker of brain cell death. HT and HT-AC inhibited LDH efflux in a concentration-dependent manner, with 50% inhibitory concentrations of 77.78 and 28.18 microM, respectively. Other well-known antioxidants such as vitamin E and N-acetyl-cysteine had no neuroprotective effect in this experimental model. After 1 week of treatment, HT (5 and 10 mg/kg per day p.o.) reduced LDH efflux by 37.8% and 52.7%, respectively, and HT-AC reduced LDH efflux by 45.4% and 67.8%. These data are additional evidence of the cytoprotective effect of VOO administration, and provide a preliminary basis for further study of these polyphenols as potential neuroprotective compounds.
羟基酪醇（HT）和羟基酪醇乙酸酯（HT-AC）是初榨橄榄油（VOO）中存在的两种具有抗氧化特性的著名酚类化合物。由于VOO已在大鼠中显示出神经保护作用，本研究的目的是在体外孵育这些化合物后或每天口服5或10 mg/kg共7天后，研究HT和HT-AC在大鼠脑片缺氧复氧模型中可能的神经保护作用。测量乳酸脱氢酶（LDH）向孵育培养基中的流出作为脑细胞死亡的标志物。HT和HT-AC以浓度依赖性方式抑制LDH流出，50%抑制浓度分别为77.78和28.18 microM。其他著名的抗氧化剂如维生素E和N-乙酰半胱氨酸在该实验模型中没有神经保护作用。治疗1周后，HT（每天口服5和10 mg/kg）分别使LDH流出减少37.8%和52.7%，HT-AC使LDH流出减少45.4%和67.8%。这些数据是VOO给药具有细胞保护作用的额外证据，并为进一步研究这些多酚作为潜在的神经保护化合物提供了初步依据。
