Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Ibaraki, Japan.
Research and Development, Kohaku Bio Technology Co., Ltd., Tsukuba 305-8572, Ibaraki, Japan.
Molecules. 2022 Mar 10;27(6):1817. doi: 10.3390/molecules27061817.
Parkinson's disease (PD) is the second most common progressive neurodegenerative disease, after Alzheimer's disease. In our previous study, we found that amber-a fossilized plant resin-can protect cells from apoptosis by decreasing the generation of reactive oxygen species (ROS). In this study, we focused on the effect of amber on 6-hydroxydopamine-induced cell apoptosis in the human neuroblastoma cell line SHSY5Y (one model for PD). Initially, we determined the protective effect of amber on the PD model. We found that amber extract has a protective effect against 6-hydroxydopamine-induced cell apoptosis. The decrease in ROS, cleaved caspase-3, pERK, and extracellular signal-regulated kinase (ERK) protein levels confirmed that amber extract decreases apoptosis via the ROS-mediated ERK signaling pathway. Furthermore, we determined the effects of amber extract on autophagy. The results showed that amber extract increased the levels of LC3II and Beclin-1, suggesting that amber extract can protect neuronal cells against 6-hydroxydopamine-induced cell apoptosis by promoting autophagy.
帕金森病（PD）是仅次于阿尔茨海默病的第二常见进行性神经退行性疾病。在我们之前的研究中，我们发现琥珀——一种化石植物树脂——可以通过减少活性氧（ROS）的产生来保护细胞免受细胞凋亡。在这项研究中，我们专注于琥珀对人神经母细胞瘤细胞系 SHSY5Y（PD 的一种模型）中 6-羟多巴胺诱导的细胞凋亡的影响。最初，我们确定了琥珀对 PD 模型的保护作用。我们发现琥珀提取物对 6-羟多巴胺诱导的细胞凋亡具有保护作用。ROS、裂解的 caspase-3、pERK 和细胞外信号调节激酶（ERK）蛋白水平的降低证实了琥珀提取物通过 ROS 介导的 ERK 信号通路减少细胞凋亡。此外，我们还确定了琥珀提取物对自噬的影响。结果表明，琥珀提取物增加了 LC3II 和 Beclin-1 的水平，这表明琥珀提取物可以通过促进自噬来保护神经元细胞免受 6-羟多巴胺诱导的细胞凋亡。
