Suzuki Satoshi, Abe Junpei, Kudo Yui, Shirai Masayuki, Kimura Ken-Ichi
General Headquarters, Misho Corporation Limited, Tokyo, Japan.
Chemical Biology Laboratory, Graduate School of Arts and Sciences, Iwate University, Morioka, Japan.
Biosci Biotechnol Biochem. 2020 Mar;84(3):518-525. doi: 10.1080/09168451.2019.1693251. Epub 2019 Nov 22.
Kuji amber is fossilized tree resin of the Late Cretaceous in Japan. In this study, new biological activities of ethanol extract of Kuji amber (EtOH ext.) and supercritical carbon dioxide fluid extract of Kuji amber (scCO ext.) were examined. Both EtOH ext. and scCO ext. inhibited melanin production in B16 mouse melanoma cells and promoted collagen production in human skin fibroblast SF-TY cells. The scCO ext. had more potent activity than that of EtOH ext. and may depend on the efficiency of the extraction. The main new biologically active compound in Kuji amber, kujigamberol had no activities against melanin production, however, it promoted collagen production at low concentrations. A biologically active compound having a different structure, spirolactone norditerpenoid, showed both the inhibition activity against melanin production and the promotion activity of collagen production in a dose dependent manner. EtOH ext. and scCO ext., which include both kujigamberol and spirolactone norditerpenoid, have not only anti-allergy activity, but also inhibit melanin production and promote collagen production.
久慈琥珀是日本晚白垩世的化石树脂。在本研究中，检测了久慈琥珀乙醇提取物（EtOH ext.）和久慈琥珀超临界二氧化碳流体提取物（scCO ext.）的新生物活性。EtOH ext.和scCO ext.均抑制B16小鼠黑色素瘤细胞中的黑色素生成，并促进人皮肤成纤维细胞SF-TY细胞中的胶原蛋白生成。scCO ext.的活性比EtOH ext.更强，这可能取决于提取效率。久慈琥珀中的主要新生物活性化合物久慈琥珀醇对黑色素生成无活性，但在低浓度下可促进胶原蛋白生成。一种结构不同的生物活性化合物螺内酯去甲二萜以剂量依赖的方式显示出对黑色素生成的抑制活性和对胶原蛋白生成的促进活性。包含久慈琥珀醇和螺内酯去甲二萜的EtOH ext.和scCO ext.不仅具有抗过敏活性，还能抑制黑色素生成并促进胶原蛋白生成。
