Li Xujian, Liu Saisai, Wang Yapeng, Lu Wei, Zhang Quanqi, Cheng Jie
Key Laboratory of Marine Genetics and Breeding, Ministry of Education, Ocean University of China, 5 Yushan Road, Qingdao 266003, China.
Laboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology, 1 Wenhai Road, Qingdao 266237, China.
Biology (Basel). 2022 Feb 23;11(3):353. doi: 10.3390/biology11030353.
The heat shock protein (Hsp) superfamily has received accumulated attention because it is ubiquitous and conserved in almost all living organisms and is involved in a wide spectrum of cellular responses against diverse environmental stresses. However, our knowledge about the Hsp co-chaperon network is still limited in non-model organisms. In this study, we provided the systematic analysis of 95 genes (s) in the genome of spotted sea bass (), an important aquaculture species in China that can widely adapt to diverse salinities from fresh to sea water, and moderately adapt to high alkaline water. Through in silico analysis using transcriptome and genome database, we determined the expression profiles of s in response to salinity change and alkalinity stress in gills. The results revealed that s were sensitive in response to alkalinity stress, and the -- members were more actively regulated than other s and may also be coordinately interacted as co-chaperons. This was in accordance with the fact that members of , , and evolved more rapidly in than other teleost lineages with positively selected sites detected in their functional domains. Our results revealed the diverse and cooperated regulation of s under alkaline stress, which may have arisen through the functional divergence and adaptive recruitment of the -- co-chaperons and will provide vital insights for the development of cultivation in alkaline water.
热休克蛋白（Hsp）超家族一直备受关注，因为它在几乎所有生物体中普遍存在且保守，并参与了细胞对各种环境压力的广泛反应。然而，我们对非模式生物中Hsp共伴侣网络的了解仍然有限。在本研究中，我们对中国重要的水产养殖品种花鲈基因组中的95个基因进行了系统分析，花鲈能够广泛适应从淡水到海水的不同盐度，并能适度适应高碱性水。通过利用转录组和基因组数据库进行电子分析，我们确定了花鲈鳃中热休克蛋白基因在盐度变化和碱度胁迫下的表达谱。结果表明，热休克蛋白基因对碱度胁迫敏感，且Hsp70家族成员比其他热休克蛋白基因受到更积极的调控，并且可能作为共伴侣协同相互作用。这与以下事实相符：Hsp70、Hsp90和Hsp10在花鲈中的进化速度比其他硬骨鱼谱系更快，在其功能域中检测到正选择位点。我们的结果揭示了碱胁迫下热休克蛋白基因的多样化和协同调控，这可能是通过Hsp70共伴侣的功能分化和适应性招募产生的，将为碱性水养殖花鲈的发展提供重要见解。
