Zámocký Marcel, Musil Miloš, Danchenko Maksym, Ferianc Peter, Chovanová Katarína, Baráth Peter, Poljovka Andrej, Bednář David
Laboratory for Phylogenomic Ecology, Institute of Molecular Biology, Slovak Academy of Sciences, Dúbravská cesta 21, SK-84551 Bratislava, Slovakia.
University of Natural Resources and Life Sciences, Vienna, Department of Chemistry, Institute of Biochemistry, Muthgasse 18, 1190 Vienna, Austria.
Biology (Basel). 2022 Mar 17;11(3):459. doi: 10.3390/biology11030459.
In this study, we focus on a detailed bioinformatics analysis of genes, mainly within the genomes of (Ascomycota, Leotiomycetes), which is a specifically evolved fungal family of necrotrophic host generalists and saprophytic or biotrophic host specialists. Members of the genus produce only sclerotia and no fruiting bodies or spores. Thus, their physiological role for peroxidases remains open. A representative species, is a dangerous plant pathogen causing white rot in species, particularly in onions, leeks, and garlic. On a worldwide basis, the white rot caused by this soil-borne fungus is apparently the most serious threat to -crop production. We have also found very similar peroxidase sequences in the related fungus , although with minor yet important modifications in the architecture of its active centre. The presence of specific mRNA was confirmed by transcriptomic analysis. The presence of Hybrid B peroxidase at the protein level as the sole extracellular peroxidase of this fungus was confirmed in the secretome of through detailed proteomic analyses. This prompted us to systematically search for all available genes coding for Hybrid B heme peroxidases in the whole fungal family of . We present here a reconstruction of their molecular phylogeny and analyse the unique aspects of their conserved-sequence features and structural folds in corresponding ancestral sequences.
在本研究中，我们着重对基因进行详细的生物信息学分析，主要针对（子囊菌门，柔膜菌纲）的基因组，这是一个经过特殊进化的真菌家族，包含坏死性寄主泛养菌以及腐生或生物营养型寄主专养菌。该属成员仅产生菌核，不产生子实体或孢子。因此，它们过氧化物酶的生理作用仍不明确。一个代表性物种是一种危险的植物病原体，会在多种物种中引发白腐病，尤其是洋葱、韭菜和大蒜。在全球范围内，这种土壤传播真菌引起的白腐病显然是对作物生产最严重的威胁。我们在相关真菌中也发现了非常相似的过氧化物酶序列，尽管其活性中心结构有微小但重要的修饰。通过转录组分析证实了特定mRNA的存在。通过详细的蛋白质组分析，在该真菌的分泌组中证实了杂交B过氧化物酶作为该真菌唯一的细胞外过氧化物酶在蛋白质水平上的存在。这促使我们系统地搜索整个真菌家族中所有编码杂交B血红素过氧化物酶的可用基因。我们在此展示了它们的分子系统发育重建，并分析了其保守序列特征和相应祖先序列中结构折叠的独特方面。
