Centre for Organismal Studies Heidelberg, Heidelberg University, Heidelberg, Germany; Institute of Plant Sciences, University of Bern, Bern, Switzerland.
Department of Plant Sciences, University of California, Davis, CA, United States; Howard Hughes Medical Institute, Chevy Chase, MD, United States.
Curr Top Dev Biol. 2022;147:33-71. doi: 10.1016/bs.ctdb.2021.12.012. Epub 2022 Mar 14.
The arrival of cheap and high-throughput sequencing paired with efficient gene editing technologies allows us to use non-traditional model systems and mechanistically approach biological phenomena beyond what was conceivable just a decade ago. Venturing into different model systems enables us to explore for example clade-specific environmental responses to changing climates or the genetics and development of clade-specific organs, tissues and cell types. We-both early career researchers working with the wild grass model Brachypodium distachyon-want to use this review to (1) highlight why we think B. distachyon is a fantastic grass developmental model system, (2) summarize the tools and resources that have enabled discoveries made in B. distachyon, and (3) discuss a handful of developmental biology vignettes made possible by using B. distachyon as a model system. Finally, we want to conclude by (4) relating our personal stories with this emerging model system and (5) share what we think is important to consider before starting work with an emerging model system.
廉价、高通量的测序技术与高效的基因编辑技术相结合,使我们能够使用非传统的模式系统,从机制上研究十年前难以想象的生物学现象。进入不同的模式系统使我们能够探索例如特定进化枝对气候变化的环境响应,或者特定进化枝的器官、组织和细胞类型的遗传学和发育。我们——两位从事野生草模型拟南芥研究的早期职业研究人员——希望利用这篇综述(1)强调为什么我们认为拟南芥是一个极好的草类发育模式系统,(2)总结使在拟南芥中取得发现成为可能的工具和资源,以及(3)讨论通过使用拟南芥作为模型系统而成为可能的一些发育生物学实例。最后,我们想通过(4)讲述我们与这个新兴模式系统的个人故事,以及(5)分享在开始使用新兴模式系统之前我们认为需要考虑的重要事项来结束。