Department of Smart Green Technology Engineering, Pukyong National University, Busan 48513, Republic of Korea; Department of Display Engineering, Pukyong National University, Busan 48513, Republic of Korea.
Department of Biomedical Engineering, Pukyong National University, Busan 48513, Republic of Korea.
Carbohydr Polym. 2022 Jun 15;286:119303. doi: 10.1016/j.carbpol.2022.119303. Epub 2022 Mar 1.
One of the most promising strategies for the controlled release of therapeutic molecules is stimuli-responsive and biodegradable hydrogels developed from natural polymers. However, current strategies to development stimuli-responsive hydrogels lack precise control over drug release profile and use cytotoxic materials during preparation. To address these issues, multi-stimuli responsive hydrogels derived from hyaluronic acid and diselenide based cross-linker were developed for the controlled release of doxorubicin (DOX). Hydrogels were rapidly formed via an inverse electron demand Diels-Alder click chemistry and encapsulated DOX/indocyanine green (ICG) in their porous networks. The hydrogels showed a rapid release of DOX in acidic (pH 5), reducing (10 mmol DTT), and oxidizing medium (0.5% HO), and after NIR irradiation. The in vitro experiments demonstrated that hydrogels were highly cytocompatible and the DOX-loaded hydrogels induced similar anti-tumor effect as compared to that of the free-DOX. Furthermore, DOX + ICG loaded hydrogels increased the antitumor efficacy of DOX after NIR irradiation.
一种有前途的治疗分子控制释放策略是使用天然聚合物开发的对刺激响应和可生物降解的水凝胶。然而，目前开发对刺激响应水凝胶的策略缺乏对药物释放曲线的精确控制，并且在制备过程中使用细胞毒性材料。为了解决这些问题，开发了源自透明质酸和二硒键交联剂的多刺激响应水凝胶，用于阿霉素（DOX）的控制释放。水凝胶通过逆电子需求 Diels-Alder 点击化学快速形成，并将 DOX/吲哚菁绿（ICG）封装在其多孔网络中。水凝胶在酸性（pH 5）、还原（10 mmol DTT）和氧化介质（0.5% HO）中以及在近红外辐射后迅速释放 DOX。体外实验表明，水凝胶具有高度的细胞相容性，负载 DOX 的水凝胶的抗肿瘤效果与游离 DOX 相似。此外，负载 DOX+ICG 的水凝胶在近红外辐射后增加了 DOX 的抗肿瘤功效。
