Division of Biotechnology, Biology Department, Faculty of Science, Istanbul University, Vezneciler, 34134 Istanbul, Turkey.
Department of Medical Microbiology, Istanbul University-Cerrahpasa, Cerrahpaşa, 34320 Istanbul, Turkey.
Sci Total Environ. 2022 Aug 1;832:154716. doi: 10.1016/j.scitotenv.2022.154716. Epub 2022 Mar 23.
This study aimed to explore the effect of a bacteriophage cocktail, pyophage, on the treatment of wastewater containing antibiotics in an anaerobic membrane bioreactor (AnMBR). During the operational period, performance of the AnMBR was monitored through the changes in chemical oxygen demand (COD), antibiotic removal, transmembrane pressure, and biogas production. Microbial community structure and composition, as well as the occurrence of antibiotic resistance genes were analyzed through shotgun metagenomics analysis. When exposed to pyophage, COD removal efficiency was enhanced up to 96%, whereas membrane fouling was delayed by 25%. Average biogas production was doubled from 224.2 mL/d in control with antibiotics to 447.3 mL/d when exposed to pyophage cocktail with considerable alterations to the archaeal and bacterial community structures. Most notably, the methanogenic community shifted from dominance of Methanothermobacter to Methanoculleus, along with syntrophic bacteria. The results provide insight into the synergistic effects of phage-bacteria and methanogenic communities and illustrate the potential of bacteriophages as bio-enhancers.
本研究旨在探讨噬菌体鸡尾酒 Pyophage 对厌氧膜生物反应器 (AnMBR) 中含抗生素废水处理的影响。在运行期间，通过化学需氧量 (COD)、抗生素去除率、跨膜压力和沼气产量的变化来监测 AnMBR 的性能。通过鸟枪法宏基因组分析，分析了微生物群落结构和组成以及抗生素抗性基因的发生情况。当暴露于 Pyophage 时，COD 去除效率提高了 96%，而膜污染则延迟了 25%。当暴露于噬菌体鸡尾酒时，平均沼气产量从对照（含抗生素）的 224.2 mL/d 增加到 447.3 mL/d，这使得古菌和细菌群落结构发生了相当大的变化。值得注意的是，产甲烷群落从 Methanothermobacter 向 Methanoculleus 和共栖细菌的优势转变。研究结果深入了解了噬菌体-细菌和产甲烷群落的协同作用，并说明了噬菌体作为生物增强剂的潜力。
