Queen Mary University of London, London, UK.
Evid Based Dent. 2022 Mar;23(1):30-31. doi: 10.1038/s41432-022-0249-8. Epub 2022 Mar 25.
Data sources Four electronic databases were searched: Medline (OVID), Web of Science, Embase and Scopus. An initial search was carried out in May 2018, and this was updated in September 2020. There was no time restriction on the studies included, and the final data consisted of studies published from 2004-2020.Study selection The electronic database search yielded 2,764 abstracts, and following de-duplication, 1,873 articles were screened in accordance with the exclusion criteria. In total, 346 articles were selected for full-text screening by four pairs of blinded reviewers and 295 articles were included in the final study. The main objectives of this study were to investigate a suitable biomarker for early detection of oral squamous cell carcinoma (OSCC) and oral potentially malignant disorders (OPMDs), and to assess the relationships between salivary biomarkers and risk factors for OSCC and OPMD. The Newcastle-Ottawa Scale was used for quality assessment. Most studies were considered to have a moderate risk of bias. The publications included fulfilled the following criteria: original research, human subjects with oral cavity cancer, OSCC or OPMD, aged 18 years or over, studies analysing biomarkers in saliva or salivary rinse, and studies published in English.Data extraction and synthesis Data extraction followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guideline process. The following data parameters were included in the studies chosen for assessment: study design, first author, year of publication, country of study population, age, sample size, gender, salivary biomarkers, method used to analyse the biomarkers, relationships between risk factors and salivary biomarkers, and conclusions.Results Following evaluation of 295 articles and selection of suitable salivary biomarkers, 28 articles were chosen to further assess interleukins as potential biomarkers and 33 studies were found to report a relationship between salivary biomarkers and risk factors. From the data reported, IL1β, IL6 and IL8 were identified as being statistically significant and most suitable for early identification of OSCC and OPMDs. In smokers, there were significant differences found in certain biomarkers compared to controls. There were statistically non-significant relationships found between biomarkers and alcohol, as well as other risk factors.Conclusion The authors proposed that a proteomic salivary biomarker panel, including a combination of IL1β, IL6 and IL8, would be suitable for clinical validation for the early detection and screening of OPMDs and OSCC. They have also highlighted the presence of research gaps in the relationship between salivary biomarkers and risk factors for OPMDs and OSCC, and the need for further research to understand the role of biomarkers in disease initiation and progression.
数据来源 检索了四个电子数据库：Medline（OVID）、Web of Science、Embase 和 Scopus。2018 年 5 月进行了首次搜索，并于 2020 年 9 月进行了更新。研究没有时间限制，最终数据包括 2004 年至 2020 年发表的研究。研究选择 电子数据库搜索产生了 2764 篇摘要，经过去重后，根据排除标准筛选了 1873 篇文章。共有四对盲审评审员对 346 篇文章进行了全文筛选，最终有 295 篇文章纳入研究。本研究的主要目的是寻找一种合适的生物标志物，用于早期检测口腔鳞状细胞癌（OSCC）和口腔潜在恶性疾病（OPMDs），并评估唾液生物标志物与 OSCC 和 OPMD 风险因素之间的关系。纽卡斯尔-渥太华量表用于质量评估。大多数研究被认为存在中度偏倚风险。符合以下标准的出版物均被纳入研究：原始研究、口腔癌症、OSCC 或 OPMD 的人类受试者、年龄 18 岁或以上、分析唾液或唾液冲洗液中生物标志物的研究、以及用英文发表的研究。数据提取和综合 数据提取遵循系统评价和荟萃分析的首选报告项目（PRISMA）指南流程。纳入评估的研究包括以下数据参数：研究设计、第一作者、发表年份、研究人群所在国家、年龄、样本量、性别、唾液生物标志物、用于分析生物标志物的方法、风险因素与唾液生物标志物之间的关系，以及结论。结果 在评估了 295 篇文章并选择了合适的唾液生物标志物后，选择了 28 篇文章进一步评估白细胞介素作为潜在的生物标志物，发现 33 项研究报告了唾液生物标志物与风险因素之间的关系。从报告的数据中，发现 IL1β、IL6 和 IL8 具有统计学意义，最适合早期识别 OSCC 和 OPMDs。在吸烟者中，与对照组相比，某些生物标志物存在显著差异。在生物标志物与酒精以及其他风险因素之间发现无统计学意义的关系。结论 作者提出，一种包括 IL1β、IL6 和 IL8 的蛋白质组唾液生物标志物组合，将适合用于 OPMDs 和 OSCC 的早期检测和筛查的临床验证。他们还强调了在 OPMDs 和 OSCC 的唾液生物标志物与风险因素之间的关系方面存在研究空白，需要进一步研究以了解生物标志物在疾病发生和发展中的作用。
