Pediatric Department, Faculty of Medicine, Assiut University, Assiut, 71516, Egypt.
Clinical Pathology Department, Faculty of Medicine, Assiut University, Assiut, 71516, Egypt.
Ir J Med Sci. 2023 Feb;192(1):403-407. doi: 10.1007/s11845-022-02983-2. Epub 2022 Mar 26.
Meningitis is one of the most dangerous infection affecting children. The need for rapid and accurate diagnosis is mandatory for improving the outcome.
To evaluate the role of multiplex polymerase chain reaction (PCR) in diagnosis of meningitis either bacterial or viral and to detect its accuracy.
A cross-sectional study was carried out in University Children Hospital, Faculty of Medicine, between November 2019 and September 2020. The study was approved by the Ethics Review Board of Faculty of Medicine, Assiut University, and informed written consent was obtained. The committee's reference number is 17200161. Clinicaltrails.gov ID: NCT03387969. Forty-eight children aged 2 to 18 years with meningitis were included. Detailed history and examination, blood glucose level at time of admission prior to lumbar puncture, and multiplex PCR in cerebrospinal fluid (CSF) were evaluated.
The mean age of children was 3.27 ± 1.27 years. Thirty-five (72.9%) cases were bacterial meningitis while 13 (27.1%) cases were viral meningitis. Multiplex PCR had 94% sensitivity and 100% specificity for diagnosis of bacterial meningitis.
Multiplex PCR may help in diagnosis and differentiation of bacterial and viral meningitis with accurate and rapid results.
脑膜炎是影响儿童的最危险感染之一。为了改善预后，快速准确的诊断是必要的。
评估多重聚合酶链反应（PCR）在诊断细菌性或病毒性脑膜炎中的作用，并检测其准确性。
这是一项 2019 年 11 月至 2020 年 9 月在大学儿童医院进行的横断面研究。该研究得到了 Assiut 大学医学院伦理审查委员会的批准，并获得了书面知情同意。委员会的参考编号为 17200161。Clinicaltrails.gov ID：NCT03387969。纳入了 48 名年龄在 2 至 18 岁的脑膜炎患儿。评估了详细的病史和检查、腰椎穿刺前入院时的血糖水平以及脑脊液中的多重 PCR。
儿童的平均年龄为 3.27±1.27 岁。35 例（72.9%）为细菌性脑膜炎，13 例（27.1%）为病毒性脑膜炎。多重 PCR 对细菌性脑膜炎的诊断具有 94%的敏感性和 100%的特异性。
多重 PCR 可以帮助诊断和区分细菌性和病毒性脑膜炎，并提供准确和快速的结果。
