Wang Honggui, Gong Shujun, Li Xinhao, Chong Yang, Ge Qingfeng, Wang Jing, Zhang Ya, Liu Yuan, Jiao Xin'an
School of Environmental Science and Engineering, Yangzhou University, 225127 Yangzhou, Jiangsu, China.
School of Environmental Science and Engineering, Yangzhou University, 225127 Yangzhou, Jiangsu, China.
Colloids Surf B Biointerfaces. 2022 Jun;214:112457. doi: 10.1016/j.colsurfb.2022.112457. Epub 2022 Mar 11.
Infection caused by antibiotic-resistant bacteria is serious threat for public health, and calls for novel antibacterial agents with versatile functions. In particular, nanomaterial is one of promising candidates to fight the increasing antibiotic resistance crisis. Here, we synthesized distinct FeO@MoS@SDS nanocomposites by ultrasonication assisted SDS coating on the FeO@MoS. Photothermal investigation indicated that the FeO@MoS@SDS showed excellent and stable photothermal performance and could be a NIR-induced photothermal reagent. It also displayed superior disinfection ability of Escherichia coli (E. coli), Methicillin-resistant Staphylococcus aureus (MRSA), and Pseudomonas aeruginosa (P. aeruginosa) and in vivo wound healing ability with the help of NIR irradiation. According to the results of electron paramagnetic resonance (EPR) and radical capture tests, plenty of superoxide, hydroxyl radicals, singlet oxygen and living cell reactive oxygen species can be observed under NIR irradiation. Besides, the synergistic effect FeO@MoS@SDS and NIR irradiation eradicated almost all the biofilms of MRSA, so this kind of function enhanced the disinfection ability of FeO@MoS@SDS under NIR irradiation. Furthermore, its inhibition effect on antibiotic resistance gene dissemination was also investigated. As expected, the FeO@MoS@SDS could efficiently and broadly block the horizontal transfer of antibiotic resistance genes which mediated by conjugative plasmids, and its blocking effect was better than that we have reported FeO@MoS. Overall, our findings revealed that the FeO@MoS@SDS could be a potential candidate for photothermal-photodynamic therapy and antibiotic resistance gene dissemination inhibition.
抗生素耐药菌引起的感染对公众健康构成严重威胁，因此需要具有多种功能的新型抗菌剂。特别是，纳米材料是应对日益严重的抗生素耐药危机的有希望的候选者之一。在这里，我们通过超声辅助在FeO@MoS上包覆SDS合成了独特的FeO@MoS@SDS纳米复合材料。光热研究表明，FeO@MoS@SDS表现出优异且稳定的光热性能，可作为近红外诱导的光热试剂。在近红外照射的帮助下，它还对大肠杆菌、耐甲氧西林金黄色葡萄球菌和铜绿假单胞菌显示出卓越的消毒能力以及体内伤口愈合能力。根据电子顺磁共振（EPR）和自由基捕获测试的结果，在近红外照射下可以观察到大量的超氧阴离子、羟基自由基、单线态氧和活细胞活性氧。此外，FeO@MoS@SDS与近红外照射的协同作用几乎根除了耐甲氧西林金黄色葡萄球菌的所有生物膜，因此这种功能增强了FeO@MoS@SDS在近红外照射下的消毒能力。此外，还研究了其对抗生素耐药基因传播的抑制作用。正如预期的那样，FeO@MoS@SDS可以有效且广泛地阻断由接合质粒介导的抗生素耐药基因的水平转移，并且其阻断效果优于我们之前报道的FeO@MoS。总体而言，我们的研究结果表明，FeO@MoS@SDS可能是光热-光动力疗法和抗生素耐药基因传播抑制的潜在候选者。
