Coupling microscale zero-valent iron and autotrophic hydrogen-bacteria provides a sustainable remediation solution for trichloroethylene-contaminated groundwater: Mechanisms, regulation, and engineering implications.

Coupling microscale zero-valent iron (mZVI) and autotrophic hydrogen bacteria (AHB) has gained increasing attention owing to its potential to improve dechlorination performance by bridging H donors and acceptors. However, few studies have attempted to test its sustainable remediation performance and to comprehensively unveil the governing mechanisms. This study systematically compared the performances of different systems (mZVI, H-AHB, and mZVI-AHB) for trichloroethylene (TCE) removal, and further optimized dechlorination and H evolution of mZVI-AHB synchronously by regulating the mZVI particle size and dosage to achieve a win-win remediation solution. The final removal efficiency and removal rate of TCE by mZVI-AHB were 1.67-fold and 5.30-fold of those by mZVI alone respectively, and mZVI-AHB resulted in more complete dechlorination than H-AHB alone. Combining H evolution kinetics, material characterization data, and bacterial community analysis results, the improved dechlorination performance of mZVI-AHB was mainly due to the following mechanisms: H generated by mZVI corrosion was efficiently utilized by AHB, lasting corrosion of mZVI was facilitated by AHB, and dechlorination functional bacteria were highly enriched by mZVI. Finally, the remediation performance of mZVI-AHB with different mZVI particle sizes and dosages was evaluated comprehensively in terms of dechlorination reactivity, H utilization efficiency and chemical cost, and suggestions for possible engineering applications are provided.