Department of Food Science & Biotechnology, and Carbohydrate Bioproduct Research Center, Sejong University, Seoul 05006, Republic of Korea.
Department of Agricultural Biotechnology, and Center for Agricultural Microorganism and Enzyme, Seoul National University, Seoul 08826, Republic of Korea.
N Biotechnol. 2022 Sep 25;70:1-8. doi: 10.1016/j.nbt.2022.03.004. Epub 2022 Mar 23.
The aim of this study was to establish an efficient bioprocess for the synthesis of trehalulose as a novel sweetener. This disaccharide has 70% of the sweetness of sucrose and bioactive properties such as anti-cariogenicity and anti-oxidizing activity. In this study, amylosucrase from the Deinococcus deserti (DdAS) gene was expressed and purified. When DdAS was reacted with 2 M sucrose at 35 °C for 120 h, the yield ratio of trehalulose to turanose was approximately 2:1. The trehalulose yield increased when extrinsic fructose was added. Under optimum conditions for trehalulose synthesis, the yield reached 36% (246 g/L, sucrose basis) starting with 2 M sucrose + 0.75 M fructose and showed the highest trehalulose productivity (1.94 g/L/h). As a result, a novel amylosucrase that synthesized trehalulose as the major product was developed, in contrast to other studied amylosucrase-type enzymes. DdAS could be utilized industrially in a bioprocess for producing trehalulose as a functional sucrose alternative.
本研究旨在建立一种高效的生物过程，以合成新型甜味剂海藻糖。这种二糖的甜度是蔗糖的 70%，具有抗龋齿和抗氧化活性等生物活性。在本研究中，表达和纯化了来自 Deinococcus deserti 的淀粉蔗糖酶（DdAS）基因。当 DdAS 与 2 M 蔗糖在 35°C 下反应 120 h 时，海藻糖与棉子糖的产率比约为 2:1。添加外源性果糖可提高海藻糖的产率。在最佳海藻糖合成条件下，以 2 M 蔗糖+0.75 M 果糖为起始原料，产率达到 36%（246 g/L，以蔗糖计），表现出最高的海藻糖生产率（1.94 g/L/h）。因此，与其他研究的淀粉蔗糖酶相比，开发了一种新型的以海藻糖为主要产物的淀粉蔗糖酶。DdAS 可在生产海藻糖作为功能性蔗糖替代品的生物过程中在工业上得到利用。
