Zhao Shunan, Wu Yuehan, Yao Yilin, Li Jingyi, Niu Qigui
School of Environmental Science and Engineering, China-America CRC for Environment & Health of Shandong Province, Shandong University, 72# Jimo Binhai Road, Qingdao, Shandong, 266237, China; School of Environment, Center for Water and Ecology, State Key Joint Laboratory of Environment Simulation and Pollution Control, Tsinghua University, 30# Haidian Shuangqing Road, Beijing, 100084, China.
School of Environmental Science and Engineering, China-America CRC for Environment & Health of Shandong Province, Shandong University, 72# Jimo Binhai Road, Qingdao, Shandong, 266237, China.
J Environ Manage. 2022 Jun 15;312:114934. doi: 10.1016/j.jenvman.2022.114934. Epub 2022 Mar 24.
This study evaluated the inhibitory effect and mitigation strategy of dodecyl dimethyl benzyl ammonium chloride (DDBAC) suppression on anaerobic digestion. With the 12 h-suppression, only 16.64% of anaerobes were alive, and acetotrophic methanogens were significantly inhibited. As for batch test, DDBAC suppression significantly prolonged the start-up of systems and decreased the biogas production. In cellulose semi-continuous digestion process, the DDBAC suppression induced volatile fatty acids accumulation and pH decrease. However, the biochar amended reactor effectively mitigated the DDBAC suppression and achieved 370.5 mL/d·g-chemical-oxygen-demand biogas production. Moreover, 17.8% more protein in extracellular polymeric substances was secreted as the bio-barrier to defense the DDBAC suppression. Furthermore, microbial analysis showed that biochar addition selectively enriched directed interspecies electron transfer (DIET) participant bacteria (Anaerolineaceae and Syntrophomonas) and methanogens (Methanosaeta and Methanobacterium). Meanwhile, the potential metabolic pathway analysis showed that the abundance of amino acids and energy metabolism were increased 28% and 8%, respectively. The abundance of encoding enzyme related to hydrogenotrophic and acetotrophic methanogenesis enriched 1.88 times and 1.48 times, respectively. These results showed the performance and mechanisms involved in DIET establishment with ethanol stimulation biochar addition.
本研究评估了十二烷基二甲基苄基氯化铵（DDBAC）抑制对厌氧消化的抑制作用及缓解策略。经过12小时的抑制，仅有16.64%的厌氧菌存活，乙酸营养型产甲烷菌受到显著抑制。对于批次试验，DDBAC抑制显著延长了系统的启动时间并降低了沼气产量。在纤维素半连续消化过程中，DDBAC抑制导致挥发性脂肪酸积累和pH值下降。然而，添加生物炭的反应器有效缓解了DDBAC抑制，实现了370.5 mL/d·g化学需氧量的沼气产量。此外，细胞外聚合物中分泌的蛋白质增加了17.8%，作为抵御DDBAC抑制的生物屏障。此外，微生物分析表明，添加生物炭选择性地富集了直接种间电子传递（DIET）参与细菌（厌氧绳菌科和互营单胞菌属）和产甲烷菌（甲烷八叠球菌属和甲烷杆菌属）。同时，潜在代谢途径分析表明，氨基酸和能量代谢的丰度分别增加了28%和8%。与氢营养型和乙酸营养型产甲烷作用相关的编码酶丰度分别富集了1.88倍和1.48倍。这些结果表明了添加乙醇刺激生物炭建立DIET的性能和机制。
