酿酒酵母质膜中晶体阵列形成与稳定性的形态学观察

Morphological observations on the formation and stability of the crystalline arrays in the plasma membrane of Saccharomyces cerevisiae.

作者信息

Sosinsky G, Schekman R, Glaeser R M

出版信息

J Ultrastruct Mol Struct Res. 1986 Jan;94(1):37-51. doi: 10.1016/0889-1605(86)90050-9.

Abstract

Two-dimensional crystalline arrays of freeze-fracture particles are known to occur in abundant quantities in the plasma membrane of stationary state yeast cells. Although these crystalline arrays are seen only infrequently in cells during mid-exponential growth, we now observe that formation of crystalline arrays can be induced in such cells by a "metabolic starvation" protocol. Surprisingly, starvation-induced formation of crystalline patches can be prevented by inhibition of new protein synthesis during the starvation period. The size and quantity of crystalline arrays can be increased by removal of the cell wall prior to starvation. Induction of crystalline arrays in protoplasts has made it possible to investigate the surface morphology of the crystalline particles in isolated membranes as well as at the extracellular surface of intact protoplasts. The stability of isolated crystalline arrays to several detergents has been investigated and conditions have been found that result in improved morphological purity of the isolated crystalline patches.

摘要

已知二维冷冻蚀刻颗粒晶体阵列大量存在于静止状态酵母细胞的质膜中。尽管这些晶体阵列在指数生长中期的细胞中很少见到，但我们现在观察到，通过“代谢饥饿”方案可以在这类细胞中诱导晶体阵列的形成。令人惊讶的是，在饥饿期间抑制新蛋白质合成可以阻止饥饿诱导的晶体斑块形成。在饥饿前去除细胞壁可以增加晶体阵列的大小和数量。原生质体中晶体阵列的诱导使得研究分离膜中以及完整原生质体胞外表面的晶体颗粒表面形态成为可能。已经研究了分离的晶体阵列对几种去污剂的稳定性，并找到了能够提高分离的晶体斑块形态纯度的条件。