Dinopoulos A, Parnavelas J G, Eckenstein F
J Neurocytol. 1986 Oct;15(5):619-28. doi: 10.1007/BF01611861.
A monoclonal antibody against choline acetyltransferase (ChAT), the acetylcholine synthesizing enzyme, was used to identify cholinergic neurons in the nucleus of the horizontal limb of the diagonal band of Broca at the light and electron microscopic levels. ChAT-labelled somata were fusiform, triangular or round in shape and varied considerably in size. Depending on the type of the cell, one to four dendrites emerged from the soma, but an axon could rarely be seen. The nuclei of most cells were round or oval, showed invaginations and displayed prominent nucleoli. The karyoplasm of the larger fusiform and triangular neurons contained abundant organelles including parallel arrays of granular endoplasmic reticulum. The synaptic input to labelled perikarya and proximal dendrites was sparse. It consisted chiefly of asymmetrical synaptic contacts, sometimes with postjunctional densities, but a few symmetrical synapses were also noted. ChAT-positive axon terminals were not identified which suggests that axon collaterals are rare within the nucleus of the horizontal limb of the diagonal band of Broca.
一种针对胆碱乙酰转移酶（ChAT，即乙酰胆碱合成酶）的单克隆抗体，被用于在光镜和电镜水平上识别布罗卡斜角带水平支核内的胆碱能神经元。ChAT标记的胞体呈梭形、三角形或圆形，大小差异很大。根据细胞类型，从胞体发出一到四条树突，但很少能看到轴突。大多数细胞的细胞核呈圆形或椭圆形，有凹陷且核仁明显。较大的梭形和三角形神经元的核质中含有丰富的细胞器，包括平行排列的粗面内质网。标记的胞体和近端树突的突触输入稀疏。主要由不对称突触接触组成，有时有突触后致密物，但也注意到一些对称突触。未识别出ChAT阳性轴突终末，这表明在布罗卡斜角带水平支核内轴突侧支很少见。
