Program in Cellular and Molecular Medicine, Boston Children's Hospital, Boston, MA, USA.
Department of Pediatrics, Harvard Medical School, Boston, MA, USA.
Methods Mol Biol. 2022;2463:11-29. doi: 10.1007/978-1-0716-2160-8_2.
Decidual NK cells (dNK) are a unique type of NK cells found at the maternal-fetal interface during pregnancy. dNK play a key role in placental development, trophoblast invasion, and immunity to viral and bacterial infection of the placenta. dNK are the predominant leukocyte population in first trimester placental tissues and comprise around 70% of the total CD45+ leukocytes. dNK remain present throughout pregnancy but their proportion decreases to 20-40% of term placenta decidual tissue leukocytes. Investigation of dNK function throughout pregnancy is of high clinical relevance for understanding the development of placental inflammatory disorders as well as maternal-to-fetal transmission of pathogens. In this chapter, we describe in detail the methods we developed to purify dNK from first trimester and term pregnancy placental tissues. These methods are suitable to assess their protein and gene expression profiles as well as their function.
蜕膜自然杀伤细胞（dNK）是妊娠期间在母体-胎儿界面发现的一种独特类型的 NK 细胞。dNK 在胎盘发育、滋养细胞浸润以及对胎盘病毒和细菌感染的免疫中发挥关键作用。dNK 是妊娠早期胎盘组织中主要的白细胞群体，占总 CD45+白细胞的 70%左右。dNK 在整个孕期都存在，但它们的比例下降到足月胎盘蜕膜组织白细胞的 20-40%。研究 dNK 在整个孕期的功能对于理解胎盘炎症性疾病的发展以及病原体从母体向胎儿的传播具有重要的临床意义。在本章中，我们详细描述了从妊娠早期和足月妊娠胎盘组织中纯化 dNK 的方法。这些方法适用于评估它们的蛋白和基因表达谱以及它们的功能。
