Molecular Imaging Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.
PET Department, Clinical Center, National Institutes of Health, Bethesda, MD, USA.
Methods Mol Biol. 2022;2463:153-161. doi: 10.1007/978-1-0716-2160-8_11.
A Zr-oxine ex vivo cell labeling method for tracking various cells by positron emission tomography (PET) imaging has recently been developed. Zr-oxine is synthesized from oxine and Zr-chloride, which was converted from Zr-oxalate, with neutralization. To track migration of natural killer (NK) cells in vivo in real time by PET imaging, NK cells are labeled with Zr-oxine ex vivo and infused to a recipient. The labeling is performed by mixing Zr-oxine solution to NK cell suspension at room temperature, followed by washing. Care should be taken to label the cells at optimal radioactivity doses that maintain their viability and functionality. Zr-oxine labeled NK cells can be tracked for their migration and distribution by PET/computed tomography imaging for at least 7 days. Of note, this protocol is applicable to other types of cells.
一种基于 Zr-oxine 的体外细胞标记方法，用于通过正电子发射断层扫描（PET）成像来追踪各种细胞，最近已经开发出来。Zr-oxine 是由氧嗪和 Zr-氯化物合成的，Zr-氯化物是由 Zr-草酸盐经中和反应转化而来的。为了通过 PET 成像实时追踪体内自然杀伤（NK）细胞的迁移，将 Zr-oxine 进行体外标记，然后输注给受者。标记是通过将 Zr-oxine 溶液在室温下混合到 NK 细胞悬浮液中，然后进行洗涤来完成的。应注意以保持细胞活力和功能的最佳放射性活度剂量来标记细胞。Zr-oxine 标记的 NK 细胞可以通过 PET/计算机断层扫描成像至少追踪 7 天，以监测其迁移和分布。值得注意的是，该方案适用于其他类型的细胞。
