Department of Biology Education, IALS, Gyeongsang National University, Jinju, 52828, Republic of Korea.
Laboratory of Molecular Microbiology, School of Biological Sciences and Institute of Microbiology, Seoul National University, Seoul, 08826, Republic of Korea.
J Microbiol. 2022 Apr;60(4):387-394. doi: 10.1007/s12275-022-1667-1. Epub 2022 Mar 28.
A reducing system of SoxR, a regulator of redox-active molecules, was identified as rsxABCDGE gene products and RseC in Escherichia coli through genetic studies. We found that ApbE was an additional component of the reducer system. Bacterial two hybrid analysis revealed that these proteins indeed had multiple interactions among themselves. RseC and RsxB formed the core of the complex, interacting with more than five other components. RsxC, the only cytoplasmic component of the system, interacted with SoxR. It might be linked with the rest of the complex via RsxB. Membrane fractions containing the wild type complex but not the mutant complex reduced purified SoxR using NADH as an electron source. These results suggest that Rsx genes, RseC, and ApbE can form a complex using NAD(P)H to reduce SoxR.
通过遗传研究，发现 SoxR（一种调节氧化还原活性分子的调节剂）的还原系统是由 rsxABCDGE 基因产物和 RseC 组成。我们发现 ApbE 是还原系统的另一个组成部分。细菌双杂交分析表明，这些蛋白质确实彼此之间存在多种相互作用。RseC 和 RsxB 形成了复合物的核心，与其他五个以上的组件相互作用。该系统中唯一的细胞质组件 RsxC 与 SoxR 相互作用。它可能通过 RsxB 与复合物的其余部分相连。含有野生型复合物而非突变型复合物的膜部分使用 NADH 作为电子源还原纯化的 SoxR。这些结果表明，Rsx 基因、RseC 和 ApbE 可以使用 NAD(P)H 形成一个复合物来还原 SoxR。
