Eisele P H, Woodle E S, Hunter G C, Talken L, Ward R E
Lab Anim Sci. 1986 Aug;36(4):402-5.
Perioperative care and anesthetic management of donor and recipient animals are crucial factors in studies involving experimental liver transplantation in the pig. Prevention of unacceptably high morbidity and mortality in the transplant recipients requires meticulous attention to anesthesia, preoperative and postoperative care. Liver transplant surgeries were performed using 15 pairs of pigs. Six of the transplant recipients were anesthetized with halothane plus 50% nitrous oxide (N2O) and oxygen (O2), and nine with isoflurane plus 50% N2O in O2. Arterial blood pressure, total anesthetic time, time of interruption of vena cava blood flow, and fluids administered, as well as length of survival were among the parameters measured and compared for the two groups. No deaths were attributed to either anesthetic technique. However, the isoflurane group had slightly higher blood pressure intraoperatively, better long range survival, and relatively rapid recoveries when compared to the halothane group. Because of these findings and the reported low rate of isoflurane metabolism and low resultant potential for formation of toxic metabolites when compared to halothane metabolism, we have elected to use the isoflurane-50% N2O regimen for this procedure.
在涉及猪实验性肝移植的研究中，供体和受体动物的围手术期护理及麻醉管理是关键因素。要防止移植受体出现不可接受的高发病率和死亡率，就需要在麻醉、术前和术后护理方面给予精心关注。使用15对猪进行肝移植手术。6只移植受体用氟烷加50%氧化亚氮（N₂O）和氧气（O₂）麻醉，9只用异氟烷加50% N₂O和O₂麻醉。测量并比较了两组的动脉血压、总麻醉时间、腔静脉血流阻断时间、补液量以及存活时间等参数。两组均无死亡病例归因于麻醉技术。然而，与氟烷组相比，异氟烷组术中血压略高，远期存活率更高，恢复相对较快。鉴于这些发现以及与氟烷代谢相比，报道的异氟烷代谢率低且形成有毒代谢物的潜在可能性低，我们选择在该手术中使用异氟烷 - 50% N₂O方案。
