Department of Histology & Embryology, Hacettepe University, Ankara, Turkey.
Department of Histology & Embryology, Lokman Hekim University, Ankara, Turkey.
J Perinat Med. 2022 Mar 28;50(7):910-925. doi: 10.1515/jpm-2021-0593. Print 2022 Sep 27.
To evaluate umbilical cord immune cells in pregnancies with autoimmune disorders (AID) and/or methylenetetrahydrofolate reductase (MTHFR) polymorphisms.
Umbilical cords were obtained from seven AID women without MTHFR polymorphisms, eight with AID and MTHFR polymorphisms, nine with MTHFR polymorphisms, and eight with neither. Umbilical cords were assessed immunohistologcally by anti-CD4, anti-CD8, anti-CD14, anti-CD19, anti-CD21, and anti-CD56 antibodies in six umbilical cord zones: 1) arterial wall 2) periarterial zone 3) venous wall 4) perivenous zone 5) intervascular zone, and 6) subamniotic zone.
AIDs and MTHFR polymorphisms had an effect on the number and composition of CD4+ cells in the venous wall. The presence of a MTHFR polymorphism may affect the number and morphology of CD4+ cells in the subamniotic zone. CD8+ cell distribution is substantially influenced by the presence of maternal risk factors. The co-existence of AID with MTHFR polymorphism has a prominent effect on the number and morphology of CD14+ cells, especially in the arterial wall. CD19+ cells were only observed in the control group in the venous wall, perivenous zone, and intervascular zone. CD21+ cells were only observed in the arterial wall of the control group and the intervascular zone of the AID group with different morphologic features. The number and morphology of CD56+ cells is prominently affected by the presence of maternal risk factors.
Umbilical cord stem cell and immune cell composition may be affected by the presence of risk factors like MTHFR polymorphisms and/or AID.
评估伴有自身免疫性疾病（AID）和/或亚甲基四氢叶酸还原酶（MTHFR）多态性的妊娠中脐带免疫细胞。
从 7 名无 MTHFR 多态性的 AID 女性、8 名 AID 和 MTHFR 多态性的女性、9 名 MTHFR 多态性的女性和 8 名既无 AID 也无 MTHFR 多态性的女性中获取脐带。通过抗 CD4、抗 CD8、抗 CD14、抗 CD19、抗 CD21 和抗 CD56 抗体对脐带进行免疫组织化学评估，脐带分为 6 个区域：1）动脉壁 2）动脉周围区 3）静脉壁 4）静脉周围区 5）血管间区和 6）羊膜下区。
AID 和 MTHFR 多态性对静脉壁中 CD4+细胞的数量和组成有影响。MTHFR 多态性的存在可能影响羊膜下区 CD4+细胞的数量和形态。CD8+细胞的分布主要受母体危险因素的影响。AID 与 MTHFR 多态性共存对 CD14+细胞的数量和形态有显著影响，尤其是在动脉壁。仅在对照组的静脉壁、静脉周围区和血管间区观察到 CD19+细胞。仅在对照组的动脉壁和 AID 组的血管间区观察到 CD21+细胞，且形态不同。CD56+细胞的数量和形态明显受母体危险因素的影响。
脐带干细胞和免疫细胞组成可能受 MTHFR 多态性和/或 AID 等危险因素的影响。
