Islam Md Atiqual, Lee Jinsol, Yoo Sang-Ho
Department of Food Science & Biotechnology, and Carbohydrate Bioproduct Research Center, Sejong University, Seoul 05006, Republic of Korea.
Food Chem. 2022 Aug 30;386:132797. doi: 10.1016/j.foodchem.2022.132797. Epub 2022 Mar 23.
The objective of this study was to investigate the effect of oximation reagents in simultaneous analysis of mono and di-saccharides using gas chromatography. Sugar oximation with O-ethylhydroxylamine separated all the mono- and di-saccharides while hydroxylamine and O-benzylhydroxylamine could make most of the saccharides separable except for xylose and arabinose. Resolution of xylose: arabinose, galactose: glucose, and fructose: galactose oximated by O-ethylhydroxylamine in DB-1ms column were 1.66, 2.15, and 6.19, respectively, which were above 1.5 and were officially acceptable for quantitative analysis according to the AOAC guideline. The applied method was then verified by the method validation parameters; LOD (0.011-0.02 mg/100 g), LOQ (0.032-0.061 mg/100 g), linearity (R = 0.9991-1.0000) and precision (repeatability RSD: 1.4-3.3%, reproducibility RSD: 1.7-7.6%). The greatest amounts of xylose (19.03 ± 0.38 mg/100 g), maltose (6,274.48 ± 173.59 mg/100 g) were found in the oyster sauce, and the contents of glucose (10,565.00 ± 125.31 mg/100 g), galactose (170.40 ± 4.62 mg/100 g) were greatest in soybean paste.
本研究的目的是调查肟化试剂在使用气相色谱法同时分析单糖和双糖中的作用。用O - 乙基羟胺进行糖肟化可分离所有单糖和双糖，而羟胺和O - 苄基羟胺除木糖和阿拉伯糖外可使大多数糖类分离。在DB - 1ms柱中，用O - 乙基羟胺肟化的木糖：阿拉伯糖、半乳糖：葡萄糖和果糖：半乳糖的分离度分别为1.66、2.15和6.19，均高于1.5，根据美国官方分析化学家协会（AOAC）指南，这在定量分析中是正式可接受的。然后通过方法验证参数对应用方法进行验证；检测限（LOD，0.011 - 0.02 mg/100 g）、定量限（LOQ，0.032 - 0.061 mg/100 g）、线性（R = 0.9991 - 1.0000）和精密度（重复性相对标准偏差：1.4 - 3.3%，再现性相对标准偏差：1.7 - 7.6%）。在蚝油中发现木糖含量最高（19.03±0.38 mg/100 g），麦芽糖含量最高（6274.48±173.59 mg/100 g），在大豆酱中葡萄糖含量最高（10565.00±125.31 mg/100 g），半乳糖含量最高（170.40±4.62 mg/100 g）。
