Ramnietse V E, Skard I V, Popova M V
Mikrobiologiia. 1978 May-Jun;47(3):430-5.
Losses of potassium ions, nucleotides and protein substances were studied upon rehydration of Saccharomyces cerevisiae cells grown on a defined nutrient medium with ethanol and glutamic acid (0.075%) and subjected to dehydration. Considerable amounts of K+ (75--82% of the total), nucleotides and protein substances were found to be released into distilled water upon rehydration of the dehydrated yeast cells. If a higher percentage of yeast cells survived, these lost less nucleotides and nitrogen-containing substances. Glutamic acid being added to the growth medium did not decrease specifically the permeability of the dehydrated yeast cells upon rehydration.
对在含有乙醇和谷氨酸(0.075%)的特定营养培养基上生长并经历脱水处理的酿酒酵母细胞进行复水时,研究了钾离子、核苷酸和蛋白质物质的损失情况。发现相当数量的钾离子(占总量的75%-82%)、核苷酸和蛋白质物质在脱水酵母细胞复水时释放到蒸馏水中。如果存活的酵母细胞比例较高,这些细胞损失的核苷酸和含氮物质就较少。向生长培养基中添加谷氨酸并不会特异性降低脱水酵母细胞复水时的通透性。