Robert-Peillard Fabien, El Mouchtari El Mountassir, Bonne Damien, Humbel Stéphane, Boudenne Jean-Luc, Coulomb Bruno
Aix Marseille Univ, CNRS, LCE, Marseille, France.
Aix Marseille Université, CNRS, Centrale Marseille iSm2, Marseille, France.
Spectrochim Acta A Mol Biomol Spectrosc. 2022 Jul 5;275:121170. doi: 10.1016/j.saa.2022.121170. Epub 2022 Mar 22.
A new microplate analytical procedure is described for the determination of nickel (Ni) ions in natural water samples. A lophine analogue fluorescent sensor was synthesized and a spectral study showed a selective fluorescence quenching effect of chemical sensor by Ni under optimized conditions. Density functional theory (DFT) calculations confirmed the formation of a Ni(II)L complex obtained by the Job plot. The calculations showed that the fluorescence emission peak of L collapses due to the distortion of L in the complex. The simple and fast microplate procedure allowed us to quantify Ni with a linear response from 1.6 to 40 µg L and a quantification limit of 5.4 µg L without the need of a preconcentration step. The optimized procedure using high-throughput microplate assay has been applied for the determination of Ni in natural water samples with good analytical performances.
本文描述了一种用于测定天然水样中镍（Ni）离子的新型微孔板分析方法。合成了一种洛粉碱类似物荧光传感器，光谱研究表明，在优化条件下，该化学传感器对镍具有选择性荧光猝灭作用。密度泛函理论（DFT）计算证实了通过乔布图得到的Ni(II)L配合物的形成。计算表明，配合物中L的畸变导致L的荧光发射峰消失。这种简单快速的微孔板方法使我们能够在无需预浓缩步骤的情况下，对镍进行定量分析，线性响应范围为1.6至40μg/L，定量限为5.4μg/L。使用高通量微孔板分析的优化方法已应用于天然水样中镍的测定，具有良好的分析性能。
