如何提高传统溶解动态核极化(dDNP)装置的效率:与流体路径兼容的dDNP/LOD-ESR探头的设计与性能
How to improve the efficiency of a traditional dissolution dynamic nuclear polarization (dDNP) apparatus: Design and performance of a fluid path compatible dDNP/LOD-ESR probe.
作者信息
Lê Thanh Phong, Hyacinthe Jean-Noël, Capozzi Andrea
机构信息
Geneva School of Health Sciences, HES-SO University of Applied Sciences and Arts Western Switzerland, Avenue de Champel 47, 1206 Geneva, Switzerland; LIFMET, Institute of Physics, École polytechnique fédérale de Lausanne (EPFL), Station 6, 1015 Lausanne, Switzerland.
Geneva School of Health Sciences, HES-SO University of Applied Sciences and Arts Western Switzerland, Avenue de Champel 47, 1206 Geneva, Switzerland; Image Guided Interventions Laboratory, Department of Radiology and Medical Informatics, University of Geneva, Rue Gabrielle-Perret-Gentil 4, 1211 Geneva, Switzerland.
出版信息
J Magn Reson. 2022 May;338:107197. doi: 10.1016/j.jmr.2022.107197. Epub 2022 Mar 16.
Dissolution Dynamic Nuclear Polarization (dDNP) was invented almost twenty years ago. Ever since, hardware advancement has observed 2 trends: the quest for DNP at higher field and, more recently, the development of cryogen free polarizers. Despite the DNP community is slowly migrating towards "dry" systems, many "wet" polarizers are still in use. Traditional DNP polarizers can use up to 100 L of liquid helium per week, but are less sensitive to air contamination and have higher cooling power. These two characteristics make them very versatile when it comes to new methods development. In this study we retrofitted a 5 T/1.15 K "wet" DNP polarizer with the aim of improving cryogenic and DNP performance. We designed, built, and tested a new DNP insert that is compatible with the fluid path (FP) technology and a LOgitudinal Detected Electron Spin Resonance (LOD-ESR) probe to investigate radical properties at real DNP conditions. The new hardware increased the maximum achievable polarization and the polarization rate constant of a [1-C]pyruvic acid-trityl sample by a factor 1.5. Moreover, the increased liquid He holding time together with the possibility to constantly keep the sample space at low pressure upon sample loading and dissolution allowed us to save about 20 L of liquid He per week.
溶解动态核极化(dDNP)发明于近二十年前。从那时起,硬件发展呈现出两种趋势:追求更高场强下的DNP,以及最近无液氦极化器的发展。尽管DNP领域正慢慢向“干式”系统转变,但许多“湿式”极化器仍在使用。传统的DNP极化器每周最多可消耗100升液氦,但对空气污染不太敏感且具有更高的冷却功率。这两个特性使得它们在新方法开发方面非常通用。在本研究中,我们对一台5T/1.15K的“湿式”DNP极化器进行了改造,目的是提高低温性能和DNP性能。我们设计、制造并测试了一种与流体路径(FP)技术兼容的新型DNP插入件以及一个纵向检测电子自旋共振(LOD-ESR)探头,以研究实际DNP条件下的自由基特性。新硬件使[1-C]丙酮酸-三苯甲基样品的最大可实现极化和极化速率常数提高了1.5倍。此外,液氦保持时间的增加以及在样品加载和溶解时能够持续将样品空间保持在低压状态,使我们每周能够节省约20升液氦。