Institute of Biomedical Sciences, Academia Sinica, Taipei 11529, Taiwan.
Department of Psychology, Fo Guang University, Yilan County 26247, Taiwan.
Front Biosci (Landmark Ed). 2022 Mar 5;27(3):81. doi: 10.31083/j.fbl2703081.
A force transducer or automatic scoring system is not sufficient to detect small or fine seizure activity. To improve previous assessments of epileptic behavior, a novel coil method was developed to detect an early behavioral marker for epileptic seizures.
The present study used the γ-aminobutyric acid (GABA) receptor antagonist pentylenetetrazol (PTZ) to induce seizure activity and epileptic behavior in mice. A coil method was used to detect motor seizures consisting of small amplitude 19-21 Hz muscle contractions.
Seizure activity in the 19-21 Hz range detected by the coil method was positively correlated with generalized clonic seizures with a kangaroo posture after PTZ administration. GABA receptor agonist valproic acid and ethosuximide decreased PTZ-induced 19-21 Hz seizure activity. The pattern of the amplitude ratio (%) of 19-21 Hz seizure activity after administration of the GABAA/C receptor antagonist picrotoxin was similar to the group that was treated with PTZ but different from the group that was treated with the nonselective muscarinic receptor agonist pilocarpine. The coil method detected 19-21 Hz seizure activity after PTZ administration. However, the force transducer method did not detect 19-21 Hz seizure activity.
The coil method was more sensitive than the force transducer method for detecting epileptic behaviors. The findings may indicate a novel behavioral marker that can be detected by the coil method to reveal epileptic seizures, thus improving our understanding of the brain mechanisms of action and specific brain waves that are associated with PTZ-induced 19-21 Hz seizure activity.
力传感器或自动评分系统不足以检测到小的或细微的癫痫发作活动。为了改进以前对癫痫行为的评估，开发了一种新的线圈方法来检测癫痫发作的早期行为标志物。
本研究使用γ-氨基丁酸（GABA）受体拮抗剂戊四氮（PTZ）诱导小鼠癫痫发作和癫痫行为。线圈方法用于检测由小振幅 19-21 Hz 肌肉收缩组成的运动性癫痫发作。
线圈方法检测到的 19-21 Hz 范围内的癫痫发作活动与 PTZ 给药后出现袋鼠姿势的全身性强直阵挛性癫痫发作呈正相关。GABA 受体激动剂丙戊酸和乙琥胺可降低 PTZ 诱导的 19-21 Hz 癫痫发作活动。给予 GABAA/C 受体拮抗剂荷包牡丹碱后，19-21 Hz 癫痫发作活动的幅度比（%）的模式与给予 PTZ 的组相似，但与给予非选择性毒蕈碱受体激动剂毛果芸香碱的组不同。线圈方法检测到 PTZ 给药后的 19-21 Hz 癫痫发作活动，但力传感器方法未检测到 19-21 Hz 癫痫发作活动。
线圈方法比力传感器方法更敏感，可用于检测癫痫行为。这些发现可能表明，线圈方法可以检测到一种新的行为标志物，从而揭示癫痫发作，从而更好地了解与 PTZ 诱导的 19-21 Hz 癫痫发作活动相关的大脑作用机制和特定脑电波。
