《宫颈不典型增生及鳞癌中细胞角蛋白 7 表达的研究及与临床病理相关性分析》

A Study of Cytokeratin-7 Expression and Clinicopathological Correlation in Dysplasia and Squamous Cell Carcinoma of the Cervix.

机构信息

Department of Pathology, M.S. Ramaiah Medical College, Rajiv Gandhi University of Health Sciences, MSR Nagar, MSRIT Post, Bengaluru, Karnataka, India.

出版信息

Asian Pac J Cancer Prev. 2022 Mar 1;23(3):885-891. doi: 10.31557/APJCP.2022.23.3.885.

DOI:10.31557/APJCP.2022.23.3.885

PMID:35345360

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9360945/

Abstract

OBJECTIVES

Cytokeratin (CK) proteins play a vital role in cancer diagnosis, of which,CK-7 is a prominent marker of squamocolumnar junction cells corresponding to the the initiating site of cervical cancer.The current study is aimed to evaluate the expression pattern of CK-7 and to corelate with the clinicopathological features in patients with cervical dysplasia and invasive squamous cell carcinoma.

METHODOLOGY

The hysterectomy and biopsy specimens from women with cervical dysplasia (n=60) and carcinoma (n=60) were evaluated histopathologically and processed for immunohistochemistry (IHC) staining to assess for CK-7 expression. The relationship between CK-7 expression and tumor characteristics like histological type of cervical intraepithelial neoplasia (CIN), tumor type and grade was evaluated. Data was analyzed using the Chi-square test ,wherein the p value ≤ 0.05 were taken for statistical significance.

RESULTS

Positive CK-7 expression was observed in 25 (41.67%) dysplasia and in 34 (56.67%) carcinoma cases. Majority of the cases were CIN III (n=31, 51.67%), large cell non-keratinizing tumor type (n=54, 90%) and moderately differentiated grade of tumor (n=52, 86.67%), out of which 18 (58.1%), 34 (62.96%) and 30 (57.69%) cases were CK-7 positive, respectively. The difference in clinical diagnosis and tumor characteristics over CK-7 expression was significant (p<0.05). The pattern of CK-7 expression in dysplasia and carcinoma cases were diffuse in 23 (38.33%) and 31 (51.67%) respectively and patchy in 2 (3.33%) and 3 (5%) of them, respectively.

CONCLUSION

Significant positive CK-7 expression in cervical dysplasia and carcinoma indicates a good clinical course and its role as a useful predictable marker for cancer progression.

摘要

目的

细胞角蛋白（CK）蛋白在癌症诊断中起着至关重要的作用，其中 CK-7 是对应宫颈癌起始部位的鳞柱状交界细胞的重要标志物。本研究旨在评估 CK-7 的表达模式，并与宫颈发育不良和浸润性鳞状细胞癌患者的临床病理特征相关联。

方法

对患有宫颈发育不良（n=60）和癌（n=60）的女性的子宫切除术和活检标本进行组织病理学评估，并进行免疫组织化学（IHC）染色以评估 CK-7 表达。评估 CK-7 表达与肿瘤特征（如宫颈上皮内瘤变（CIN）的组织学类型、肿瘤类型和分级）之间的关系。使用卡方检验分析数据，其中 p 值≤0.05 被认为具有统计学意义。

结果

在 25 例（41.67%）发育不良病例和 34 例（56.67%）癌病例中观察到 CK-7 阳性表达。大多数病例为 CIN III（n=31，51.67%）、大细胞非角化型肿瘤类型（n=54，90%）和中度分化肿瘤分级（n=52，86.67%），其中 18 例（58.1%）、34 例（62.96%）和 30 例（57.69%）的 CK-7 阳性表达。在 CK-7 表达方面，临床诊断和肿瘤特征之间的差异具有统计学意义（p<0.05）。在发育不良和癌病例中，CK-7 表达的模式分别为弥漫性 23 例（38.33%）和 31 例（51.67%），斑片状 2 例（3.33%）和 3 例（5%）。