Tsfasman I M, Nesmeianova M A, Kulaev I S
Mol Biol (Mosk). 1986 Sep-Oct;20(5):1290-8.
The suppression of some envelope proteins, localized in both the periplasm and the outer and inner membranes was shown in phoB and phoM phoR mutants of E. coli. Among these proteins are the proteins of the phosphate regulon and also those not pertaining them. As a result of phoB and phoM phoR mutations, the cytoplasmic membrane was found to be lacking in minor protein of 28,000 Mr, which belongs to the phosphate regulon. Besides, the phoM phoR mutation leads to the loss of protein of 55,000 Mr of the outer membranes, whereas phoB mutation causes loss of protein 37 000 Mr, identified as outer membrane protein OmpT. A damage in the phoB mutant of the protein proteolytic modification, probably determining the suppression of the biosynthesis of E. coli envelope secreted proteins is suggested.
在大肠杆菌的phoB和phoM phoR突变体中，发现位于周质以及外膜和内膜中的一些包膜蛋白受到抑制。这些蛋白中包括磷酸调节子的蛋白，也包括与之无关的蛋白。由于phoB和phoM phoR突变，发现细胞质膜中缺少属于磷酸调节子的28,000 Mr的小蛋白。此外，phoM phoR突变导致外膜中55,000 Mr的蛋白丢失，而phoB突变导致被鉴定为外膜蛋白OmpT的37,000 Mr的蛋白丢失。有人提出，phoB突变体中蛋白的蛋白水解修饰受损，这可能决定了大肠杆菌包膜分泌蛋白生物合成的抑制。
