活细胞成像观察抗癌药物作用下 Ca 浓度时空波动响应
Live Cell Imaging of Spatiotemporal Ca Fluctuation Responses to Anticancer Drugs.
机构信息
Department of Biochemistry, University of Colorado, Boulder, CO, USA.
出版信息
Methods Mol Biol. 2022;2488:227-236. doi: 10.1007/978-1-0716-2277-3_15.
Abstract
Synthetic triterpenoids, 2-cyano-3,12-dioxooleana-1,9-dien-28-oic acid (CDDO) and its derivatives are known to have potent anti-cancer and anti-inflammatory activities. The mechanisms of actions of CDDO and its derivatives as potential therapeutics remain elusive. Previous studies found that CDDO-Me triggers apoptosis by inducing extracellular Ca influx followed by endoplasmic reticulum (ER)-derived vacuolation. Since Ca activity in cells is dynamic and needs to be tracked in real time in living cells, we report a high-throughput and high-content imaging method to track CDDO-induced Ca fluctuation in both ER and cytosol with MATLAB script for data analysis and visualization.
摘要
合成三萜类化合物 2-氰基-3,12-二氧代齐墩果-1,9-二烯-28-酸（CDDO）及其衍生物具有很强的抗癌和抗炎活性。CDDO 及其衍生物作为潜在治疗剂的作用机制仍不清楚。先前的研究发现，CDDO-Me 通过诱导细胞外钙内流，随后引发内质网（ER）来源的空泡化，从而触发细胞凋亡。由于细胞内的钙活性是动态的，需要在活细胞中实时跟踪，因此我们报告了一种高通量和高内涵成像方法，该方法使用 MATLAB 脚本进行数据分析和可视化，可用于跟踪 ER 和细胞质中 CDDO 诱导的钙波动。
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