Jares-Erijman Elizabeth A, Jovin Thomas M
Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina.
Nat Biotechnol. 2003 Nov;21(11):1387-95. doi: 10.1038/nbt896.
Förster (or Fluorescence) Resonance Energy Transfer (FRET) is unique in generating fluorescence signals sensitive to molecular conformation, association, and separation in the 1-10 nm range. We introduce a revised photophysical framework for the phenomenon and provide a systematic catalog of FRET techniques adapted to imaging systems, including new approaches proposed as suitable prospects for implementation. Applications extending from a single molecule to live cells will benefit from multidimensional microscopy techniques, particularly those adapted for optical sectioning and incorporating new algorithms for resolving the component contributions to images of complex molecular systems.
Förster（或荧光）共振能量转移（FRET）在产生对1-10纳米范围内分子构象、缔合和分离敏感的荧光信号方面独具特色。我们为该现象引入了一个经过修订的光物理框架，并提供了适用于成像系统的FRET技术的系统目录，包括被提议作为合适实施前景的新方法。从单分子到活细胞的应用将受益于多维显微镜技术，特别是那些适用于光学切片并纳入用于解析复杂分子系统图像中各成分贡献的新算法的技术。
