He Linyan, Zhang Yawen, Hou Chunqi, Zhu Ziling, He Qi, Huang Qin, Ji Shundong
Jiangsu Institute of Hematology, National Clinical Research Center for Hematologic Diseases, Nhc Key Laboratory of Thrombosis and Hemostasis, The First Affiliated Hospital of Soochow University, Suzhou, China.
Collaborative Innovation Center of Hematology, Soochow University, Suzhou, China.
Platelets. 2022 Nov 17;33(8):1132-1138. doi: 10.1080/09537104.2022.2046723. Epub 2022 Mar 29.
Triggering receptor expressed on myeloid cells (TREM) like transcript-1 (TLT-1) is a membrane protein receptor found in α-granules of megakaryocytes and platelets. Upon platelet activation TLT-1 is rapidly relocated to the surface of platelets. In plasma, a soluble form of TLT-1 (sTLT-1) is present. Plasma levels of sTLT-1 are significantly elevated in thrombotic diseases. In the present study, we investigated to whether TLT-1 reflects platelet activation in pregnant women with preeclampsia. We studied 30 preeclamptic patients who were matched with 30 normotensive pregnant women and 30 non-pregnant controls. Basal TLT-1, P-selectin, and CD63 expressions on platelets were analyzed with the use of flow-cytometry (FCM). Platelet reactivity was induced by thrombin receptor activation peptide and determined by FCM. Plasma concentrations of sTLT-1 and soluble P-selectin (sP-selectin) were measured by an enzyme-linked immunosorbent assay. Results show that basal platelet expression of TLT-1, P-selectin and CD63 were increased in women with preeclampsia (PE) compared with normotensive pregnant women (NP). Platelets from PE women and NP women were more responsive compared to from nonpregnant women controls (NC), and which was demonstrated by increased expression of TLT-1, P-selectin, and CD63 upon stimulation . Plasma concentration of sTLT-1 was greater in PE women compared to NP women and NC women. Plasma sP-selectin level was higher in pregnant women than in nonpregnant women, but there were no significant differences between PE and NP women. In summary, our results revealed that platelet activation is prominent in preeclampsia, TLT-1 reflects platelet activation and may be a useful indicator for preeclampsia.
髓样细胞表达的触发受体（TREM）样转录本-1（TLT-1）是一种膜蛋白受体，存在于巨核细胞和血小板的α颗粒中。血小板激活后，TLT-1迅速重新定位到血小板表面。在血浆中，存在可溶性形式的TLT-1（sTLT-1）。血栓性疾病中sTLT-1的血浆水平显著升高。在本研究中，我们调查了TLT-1是否反映子痫前期孕妇的血小板激活情况。我们研究了30名单纯收缩期高血压患者，他们与30名血压正常的孕妇和30名非孕对照者相匹配。使用流式细胞术（FCM）分析血小板上的基础TLT-1、P-选择素和CD63表达。凝血酶受体激活肽诱导血小板反应性，并通过FCM测定。采用酶联免疫吸附测定法测量血浆中sTLT-1和可溶性P-选择素（sP-选择素）的浓度。结果显示，与血压正常的孕妇相比，子痫前期（PE）女性血小板上TLT-1、P-选择素和CD63的基础表达增加。与非孕女性对照（NC）相比，PE女性和NP女性的血小板反应性更高，刺激后TLT-1、P-选择素和CD63表达增加证明了这一点。与NP女性和NC女性相比，PE女性的血浆sTLT-1浓度更高。孕妇血浆sP-选择素水平高于非孕妇，但PE女性和NP女性之间无显著差异。总之，我们的结果表明，子痫前期患者血小板激活明显，TLT-1反映血小板激活，可能是子痫前期的一个有用指标。
