通过追踪 GFP 的运动对拟南芥叶片中的胞间连丝通透性进行定量分析。

Quantification of Plasmodesmata Permeability in Arabidopsis Leaves by Tracing the Movement of GFP.

机构信息

iHuman Institute, Shanghai Tech University, Shanghai, China.

Center for Plant Biology, School of Life Sciences, Tsinghua University, Beijing, China.

出版信息

Methods Mol Biol. 2022;2457:313-320. doi: 10.1007/978-1-0716-2132-5_21.

DOI:10.1007/978-1-0716-2132-5_21

PMID:35349150

Abstract

Plasmodesmata (PD) play an important role in plant growth and development and defense. The permeability of PD is strictly regulated. Here, we describe an assay for measuring the permeability of PD in Arabidopsis thaliana leaves, which relies on tracing intercellular movement of green fluorescent protein (GFP) upon transient expression of the protein-encoding plasmid delivered by particle bombardment. The method allows to assess GFP movement at single-cell resolution.

摘要

胞间连丝在植物生长发育和防御中起着重要作用。胞间连丝的通透性受到严格调控。本文描述了一种用于测量拟南芥叶片胞间连丝通透性的测定方法，该方法依赖于通过粒子轰击传递的蛋白编码质粒瞬时表达后 GFP 在细胞间的运动轨迹。该方法可在单细胞分辨率下评估 GFP 的运动。