叶片 pavement 细胞的胞间连丝通透性定量测定
Quantitative Plasmodesmata Permeability Assay for Pavement Cells of Leaves.
作者信息
Diao Min, Wang Qiannan, Huang Shanjin
机构信息
Center for Plant Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China.
iHuman Institute, Shanghai Tech University, 393 Middle Huaxia Road, Shanghai 201210, China.
出版信息
Bio Protoc. 2019 Apr 5;9(7):e3206. doi: 10.21769/BioProtoc.3206.
Plasmodesmata (PD) are intercellular channels between walled plant cells that enable the transportation of materials between adjacent cells, which are important for plant growth and development. The permeability of PD must be tightly regulated. Assays to determine the permeability of PD are crucial for related studies on the regulation of PD development and permeability. Here we describe an assay for the determination of PD permeability via the observation and quantification of GFP diffusion and cell-to-cell transport of CMV MP-GFP in leaves.
胞间连丝（PD）是有细胞壁的植物细胞之间的细胞间通道，能够使相邻细胞之间进行物质运输，这对植物的生长发育很重要。PD的通透性必须受到严格调控。测定PD通透性的实验对于PD发育和通透性调控的相关研究至关重要。在此，我们描述一种通过观察和量化叶片中绿色荧光蛋白（GFP）的扩散以及黄瓜花叶病毒运动蛋白-绿色荧光蛋白（CMV MP-GFP）的细胞间运输来测定PD通透性的实验方法。
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