基于病毒基因组的报告基因用于分析病毒运动蛋白和胞间连丝通透性。

Virus Genome-Based Reporter for Analyzing Viral Movement Proteins and Plasmodesmata Permeability.

机构信息

Department of Virology, Biological Faculty, Moscow State University, Moscow, Russia.

A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow, Russia.

出版信息

Methods Mol Biol. 2022;2457:333-349. doi: 10.1007/978-1-0716-2132-5_23.

DOI:10.1007/978-1-0716-2132-5_23

PMID:35349152

Abstract

Plant virus movement proteins (MPs) mediate cell-to-cell movement of the virus genome through plasmodesmata (PD). MPs target PD to increase their size exclusion limit (SEL), and this MP function is essential for virus intercellular trafficking. In this chapter, we describe the use of a Potato virus X genome-derived reporter for agroinfiltration-based identification of virus genome-encoded MPs and analysis of the ability of individual viral MPs or plant proteins to increase the PD SEL.

摘要

植物病毒运动蛋白（MPs）通过胞间连丝（PD）介导病毒基因组的细胞间运动。MPs 将 PD 作为靶标来增加其大小排除极限（SEL），而这种 MP 功能对于病毒细胞间运输是必不可少的。在本章中，我们描述了使用马铃薯病毒 X 基因组衍生报告基因，通过农杆菌浸润鉴定病毒基因组编码的 MPs，并分析单个病毒 MPs 或植物蛋白增加 PD SEL 的能力。