胰岛素对正常人淋巴细胞以及在体外用刀豆球蛋白A刺激后的[3H]哇巴因结合位点总数的影响。

Effects of insulin on the total number of [3H]ouabain binding sites in normal human lymphocytes and after stimulation in vitro with concanavalin A.

作者信息

Berntorp K, Ekman M, Berntorp E

出版信息

Scand J Clin Lab Invest. 1986 Oct;46(6):581-5. doi: 10.3109/00365518609083716.

Abstract

The effects of insulin in vitro on the total number of [3H]ouabain binding sites were studied in normal and concanavalin A-stimulated human T-lymphocytes after incubation for 18 and 42 h with 0, 10(2) and 10(5) mIU/l of insulin. While no effect on [3H]ouabain binding could be demonstrated in non-stimulated cells, a significant decrease could be produced in concanavalin A-stimulated cells. Mean +/- SD for the total number of [3H]ouabain binding sites per cell after 18 h incubation time with concanavalin A in the absence of insulin was 46,099 +/- 6,620 as compared with 44,783 +/- 8,347 in the presence of 10(2) mIU/l of insulin (non-significant) and 42,406 +/- 7,066 in the presence of 10(5) mIU/l (p = 0.031). The corresponding 42-h values were 47,075 +/- 9,412, 43,761 +/- 9,273 (p = 0.033) and 43,824 +/- 9,312 (p = 0.005).

摘要

在正常和伴刀豆球蛋白A刺激的人T淋巴细胞中，研究了胰岛素体外作用18小时和42小时后，胰岛素浓度分别为0、10²和10⁵ mIU/l时对[³H]哇巴因结合位点总数的影响。在未刺激的细胞中未发现对[³H]哇巴因结合有影响，但在伴刀豆球蛋白A刺激的细胞中可产生显著降低。在无胰岛素的情况下，与伴刀豆球蛋白A孵育18小时后，每个细胞的[³H]哇巴因结合位点总数的平均值±标准差为46,099±6,620，在存在10² mIU/l胰岛素时为44,783±8,347（无显著性差异），在存在10⁵ mIU/l胰岛素时为42,406±7,066（p = 0.031）。相应的42小时值分别为47,075±9,412、43,761±9,273（p = 0.033）和43,824±9,312（p = 0.005）。