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采用多重实时 PCR 和扩增子熔解曲线分析快速检测和鉴别潜在产毒的溃疡棒状杆菌和假结核棒状杆菌。

Rapid detection and discrimination of potentially toxigenic Corynebacterium ulcerans and Corynebacterium pseudotuberculosis by multiplex real-time PCR and amplicon melting curve analysis.

机构信息

Division of Bacteriology, Shimane Prefectural Institute of Public Health and Environmental Science, 582 Nishihamasada, Matsue, City, Shimane 690-0122, Japan.

Shimane Prefectural Hamada Public Health Center, 254 kataniwa, Hamada City, Shimane 697-0041, Japan.

出版信息

J Microbiol Methods. 2022 Apr;195:106454. doi: 10.1016/j.mimet.2022.106454. Epub 2022 Mar 26.

DOI:10.1016/j.mimet.2022.106454
PMID:35351489
Abstract

We developed a multiplex real-time PCR assay with amplicon melting curve analysis to rapidly discriminate Corynebacterium ulcerans from Corynebacterium pseudotuberculosis and detect the bacterial diphtheria toxin gene. This assay should be a valuable tool for identification of potentially toxigenic C. ulcerans.

摘要

我们开发了一种多重实时 PCR 检测方法，结合扩增子熔解曲线分析，可快速区分溃疡棒状杆菌和假结核棒状杆菌，并检测细菌白喉毒素基因。该检测方法应为鉴定潜在产毒溃疡棒状杆菌的有用工具。

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