Choi Young Joon, Lim Daehan, Byeon Suk Ho, Shin Eui-Cheol, Chung Hyewon
Laboratory of Immunology and Infectious Diseases, Graduate School of Medical Science and Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Korea.
Department of Ophthalmology, Ajou University School of Medicine, Suwon, Korea.
Yonsei Med J. 2022 Apr;63(4):357-364. doi: 10.3349/ymj.2022.63.4.357.
To evaluate the expression of multiple chemokine receptors in peripheral blood T cells from patients with age-related macular degeneration (AMD).
Peripheral blood mononuclear cells and/or aqueous humor were obtained from 24 AMD patients and 24 age- and sex-matched healthy controls. Chemokine receptor expression on T cells from peripheral blood was determined by multicolor flow cytometry. The levels of chemokines and cytokines in the aqueous humor from 12 AMD patients and six healthy controls were assessed.
AMD patients had increased expressions of CCR4 in CD4 T cells (=0.007) and CRTh2 in CD8 T cells (=0.002), and decreased expressions of CXCR3 in CD4 T cells (=0.029) and CXCR3, CCR5, and CXCR1 in CD8 T cells (=0.005, 0.019, and 0.007, respectively). Monocyte chemoattractant protein-1 levels were increased in the aqueous humor from AMD patients (=0.018), while the levels of interleukin (IL)-4 and IL-22 were significantly decreased compared to controls (=0.018 and 0.041, respectively).
The chemokine receptor profiles of T cells are altered in AMD patients compared to healthy controls without noticeable associations with chemokine levels in the aqueous humor. Further evaluation is needed to clarify the role of these alterations in AMD pathogenesis.
评估年龄相关性黄斑变性（AMD）患者外周血T细胞中多种趋化因子受体的表达情况。
从24例AMD患者以及24例年龄和性别匹配的健康对照者中获取外周血单个核细胞和/或房水。采用多色流式细胞术测定外周血T细胞上趋化因子受体的表达。评估了12例AMD患者和6例健康对照者房水中趋化因子和细胞因子的水平。
AMD患者CD4 T细胞中CCR4的表达增加（=0.007），CD8 T细胞中CRTh2的表达增加（=0.002），CD4 T细胞中CXCR3的表达降低（=0.029），CD8 T细胞中CXCR3、CCR5和CXCR1的表达降低（分别为=0.005、0.019和0.007）。AMD患者房水中单核细胞趋化蛋白-1水平升高（=0.018），而与对照组相比，白细胞介素（IL）-4和IL-22水平显著降低（分别为=0.018和0.041）。
与健康对照者相比，AMD患者T细胞的趋化因子受体谱发生改变，且与房水中趋化因子水平无明显关联。需要进一步评估以阐明这些改变在AMD发病机制中的作用。
