Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, Beibu Gulf University, Guangxi, Qinzhou, 535011, People's Republic of China.
Mar Biotechnol (NY). 2022 Apr;24(2):303-319. doi: 10.1007/s10126-022-10105-3. Epub 2022 Mar 30.
As an important economic shellfish, the pearl oyster, Pinctada fucata, and its larvae are an ideal model for studying molecular mechanisms of larval development in invertebrates. Larval development directly affects the quantity and quality of pearl oysters. MicroRNAs (miRNAs) may play important roles in development, but the effects of miRNA expression on P. fucata early development remain unknown. In this study, miRNA and mRNA transcriptomics of seven different P. fucata developmental stages were analyzed using Illumina RNA sequencing. A total of 329 miRNAs, including 87 known miRNAs and 242 novel miRNAs, and 33,550 unigenes, including 26,333 known genes and 7217 predicted new genes, were identified in these stages. A cluster analysis showed that the difference in the numbers of miRNAs was greatest between fertilized eggs and trochophores. In addition, the integrated mRNA transcriptome was used to predict target genes for differentially expressed miRNAs between adjacent developmental stages, and the target genes were subjected to a gene ontology enrichment analysis. Using the gene ontology annotation, 100 different expressed genes and 95 differentially expressed miRNAs were identified as part of larval development regulation. Real-time PCR was used to identify eight mRNAs and three miRNAs related to larval development. The present findings will be helpful for further clarifying the regulatory mechanisms of miRNA in invertebrate larval development.
作为一种重要的经济贝类，珍珠贝（Pinctada fucata）及其幼虫是研究无脊椎动物幼虫发育分子机制的理想模型。幼虫发育直接影响珍珠贝的数量和质量。微 RNA（miRNA）可能在发育过程中发挥重要作用，但 miRNA 表达对珍珠贝早期发育的影响尚不清楚。本研究采用 Illumina RNA 测序技术对 7 个不同珍珠贝发育阶段的 miRNA 和 mRNA 转录组进行了分析。共鉴定出 329 个 miRNA，包括 87 个已知 miRNA 和 242 个新 miRNA，以及 33550 个 unigenes，包括 26333 个已知基因和 7217 个预测新基因。聚类分析表明，miRNA 的数量差异在受精卵和担轮幼虫之间最大。此外，整合的 mRNA 转录组用于预测相邻发育阶段差异表达 miRNA 的靶基因，并对靶基因进行基因本体富集分析。使用基因本体注释，鉴定出 100 个差异表达基因和 95 个差异表达 miRNA 作为幼虫发育调控的一部分。实时 PCR 用于鉴定与幼虫发育相关的 8 个 mRNAs 和 3 个 miRNAs。本研究结果将有助于进一步阐明 miRNA 在无脊椎动物幼虫发育中的调控机制。
