环状 RNA 通过 miRNA-128-3p 减少 Sirt1/NFAT5 信号通路减轻卵清蛋白诱导的哮喘中的炎症反应
Circular RNA has Circ 001372-Reduced Inflammation in Ovalbumin-Induced Asthma Through Sirt1/NFAT5 Signaling Pathway by miRNA-128-3p.
机构信息
Department of Pediatrics, Beijing JiShuiTan Hospital, No. 68 Huinanbei Road, XinJieKou East Street, XiCheng District, Beijing, 100035, China.
出版信息
Mol Biotechnol. 2022 Sep;64(9):1034-1044. doi: 10.1007/s12033-022-00480-6. Epub 2022 Mar 30.
In this study, we sought to investigate the prospective role of circ 001372 in modifying inflammation in ovalbumin-induced asthma. In the vivo model of asthma, the serum of circ 001372 was reduced. Down-regulation of circ 001372 increased inflammation reaction (TNF-α, IL-1β, IL-6, and IL-18) and induced COX-2 and iNOS protein expression in vitro model through activation of NFAT5 and suppression of Sirt1. Up-regulation of circ 001372 decreased inflammation reaction (TNF-α, IL-1β, IL-6, and IL-18) in vitro model through inactivation of NFAT5 and induction of Sirt1 by miRNA-128-3p. The miRNA-128-3p lowered the effects of circ 001372 on inflammation in vitro model. The Sirt1 inhibitor reduced the effects of circ 001372 on inflammation in vitro model. Our results revealed the serum of circ 001372 against inflammation in ovalbumin-induced asthma through Sirt1/NFAT5 by miRNA-128-3p.
在这项研究中，我们试图研究 circ 001372 在修饰卵清蛋白诱导的哮喘中的炎症反应的前瞻性作用。在哮喘的体内模型中，circ 001372 的血清水平降低。circ 001372 的下调通过激活 NFAT5 和抑制 Sirt1，增加了体外模型中的炎症反应（TNF-α、IL-1β、IL-6 和 IL-18），并诱导 COX-2 和 iNOS 蛋白表达。circ 001372 的上调通过 miRNA-128-3p 使 NFAT5 失活并诱导 Sirt1，降低了体外模型中的炎症反应（TNF-α、IL-1β、IL-6 和 IL-18）。miRNA-128-3p 降低了 circ 001372 在体外模型中对炎症的影响。Sirt1 抑制剂降低了 circ 001372 在体外模型中对炎症的影响。我们的结果表明，血清中的 circ 001372 通过 miRNA-128-3p 介导的 Sirt1/NFAT5 途径抑制卵清蛋白诱导的哮喘中的炎症反应。
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