Seke Etet Paul F, Hamza Muaawia A, El-Tahir Ahmed, Vecchio Lorella, Osman Sayed Y, Satti Gwiria M H, Ismail Mohamed H A, Farahna Mohammed, Njamnshi Alfred K, Adem Abdu
Department of Physiological Sciences and Biochemistry, Faculty of Medicine and Biomedical Sciences, University of Garoua, Garoua, Cameroon.
Center for Sustainable Health and Development, Garoua, Cameroon.
Evid Based Complement Alternat Med. 2022 Mar 21;2022:8708961. doi: 10.1155/2022/8708961. eCollection 2022.
methanolic extract was fractionated using increasingly polar solvents. Fractions were administered to streptozotocin (STZ)-induced diabetic mice until marked motor signs developed in diabetic controls. Fine motor skills indicators were measured in the horizontal grid test (HGT) to confirm the prevention of motor disorders in treated animals. Column chromatography was used to separate the most active fraction, and subfractions were tested in turn in the HGT. Gas chromatography-mass spectrometry (GC-MS) technique was used to assess the components of the most active subfraction.
Treatment with ethyl acetate fraction and its fifth eluate (F5) preserved fine motor skills and improved the body weight and blood glucose level. At dose 1.71 mg/kg, F5 kept most parameters comparable to the nondiabetic vehicle group values. GC-MS chromatographic analysis of F5 revealed 36 compounds, the most abundantly expressed (41.8%) being the -lactam molecules -ethyl-2-carbethoxyazetidine (17.8%), ,-dimethylethanolamine (15%), and isoniacinamide (9%).
Our results suggest that subfraction F5 of extract prevented the development of motor signs and improved disease profile in an STZ-induced mouse model of diabetic encephalopathy. Antidiabetic activity of -lactam molecules accounted at least partly for these effects.
使用极性递增的溶剂对甲醇提取物进行分级分离。将各馏分给予链脲佐菌素（STZ）诱导的糖尿病小鼠，直至糖尿病对照组出现明显的运动体征。在水平网格试验（HGT）中测量精细运动技能指标，以确认治疗动物的运动障碍得到预防。采用柱色谱法分离活性最高的馏分，并依次在HGT中对亚馏分进行测试。使用气相色谱 - 质谱联用（GC - MS）技术评估活性最高的亚馏分的成分。
用乙酸乙酯馏分及其第五洗脱液（F5）进行治疗可保留精细运动技能，并改善体重和血糖水平。在剂量为1.71 mg/kg时，F5使大多数参数与非糖尿病载体组的值相当。F5的GC - MS色谱分析显示有36种化合物，其中表达最丰富的（41.8%）是β - 内酰胺分子 - 乙基 - 2 - 乙氧羰基氮杂环丁烷（17.8%）、N,N - 二甲基乙醇胺（15%）和异烟酰胺（9%）。
我们的结果表明，提取物的亚馏分F5可预防STZ诱导的糖尿病性脑病小鼠模型中运动体征的发展，并改善疾病状况。β - 内酰胺分子中的抗糖尿病活性至少部分地解释了这些作用。
