Department of Periodontics, School of Dentistry, Virginia Commonwealth University, Richmond, VA, USA.
Department of Periodontology, Peking University School and Hospital of Stomatology, National Clinical Research Center for Oral Diseases, National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing Key Laboratory of Digital Stomatology, Beijing, People's Republic of China.
J Dent Res. 2022 Aug;101(9):1110-1118. doi: 10.1177/00220345221084975. Epub 2022 Mar 31.
Human bone marrow stromal cell (hBMSC)-derived exosomes are promising therapeutics for inflammatory diseases due to their unique microRNA (miRNA) and protein cargos. Periodontal diseases often present with chronicity and corresponding exuberant inflammation, which leads to loss of tooth support. In this study, we explored whether hBMSC exosomes can affect periodontitis progression. hBMSC exosomes were isolated from cell culture medium through sequential ultracentrifugation. miRNAs and proteins that were enriched in hBMSC exosomes were characterized by RNA sequencing and protein array, respectively. hBMSC exosomes significantly suppressed periodontal keystone pathogen triggered inflammatory response in macrophages in vitro. Transcriptomic analysis suggested that exosomes exerted their effects through regulating cell metabolism, differentiation, and inflammation resolution. In vivo, weekly exosome injection into the gingival tissues reduced the tissue destruction and immune cell infiltration in rat ligature-induced periodontitis model. Collectively, these findings suggest that hBMSC-derived exosomes can potentially be used as a host modulation agent in the management of periodontitis.
人骨髓基质细胞（hBMSC）衍生的外泌体由于其独特的 microRNA（miRNA）和蛋白质负荷，成为治疗炎症性疾病的有前途的治疗方法。牙周病常表现为慢性和相应的过度炎症，导致牙齿支持组织丧失。在这项研究中，我们探讨了 hBMSC 外泌体是否会影响牙周炎的进展。通过连续超速离心从细胞培养液中分离 hBMSC 外泌体。通过 RNA 测序和蛋白质阵列分别对富含 hBMSC 外泌体的 miRNA 和蛋白质进行了表征。hBMSC 外泌体在体外显著抑制了牙周关键病原体触发的巨噬细胞炎症反应。转录组分析表明，外泌体通过调节细胞代谢、分化和炎症消退来发挥作用。在体内，每周向牙龈组织注射外泌体可减少大鼠结扎诱导的牙周炎模型中的组织破坏和免疫细胞浸润。总之，这些发现表明，hBMSC 衍生的外泌体可能可用作牙周炎管理中的宿主调节因子。
