Department of Periodontics, School of Dentistry, Virginia Commonwealth University, Richmond, VA, USA.
Methods Mol Biol. 2022;2455:333-341. doi: 10.1007/978-1-0716-2128-8_25.
Exosomes, derived from stem cells, have great promise in regenerative medicine due to their capabilities of ameliorating inflammation, preventing tissue damage and promoting healing, which in part are associated with the exosomal RNA/miRNA. The application of mesenchymal stem cell exosomes in treating hepatic disorders including nonalcoholic fatty liver disease has drawn much attention. In this chapter, we describe our experience in culturing human mesenchymal stem cells and isolating their exosomes from culture medium through ultracentrifugation. Methods to extract exosomal RNA/miRNA are also discussed.
外泌体来源于干细胞，由于其具有减轻炎症、防止组织损伤和促进愈合的能力，因此在再生医学中有很大的应用前景，而这些作用部分与外泌体 RNA/miRNA 有关。间充质干细胞外泌体在治疗肝疾病（包括非酒精性脂肪性肝病）中的应用引起了广泛关注。在本章中，我们描述了培养人源间充质干细胞并通过超速离心从培养上清液中分离其外泌体的经验。还讨论了提取外泌体 RNA/miRNA 的方法。
